cDNA microarray analysis of changes in gene expression induced by neuronal hypoxia in vitro

K. Jin, X. O. Mao, M. W. Eshoo, G. Del Rio, R. Rao, D. Chen, R. P. Simon, D. A. Greenberg

Research output: Contribution to journalArticle

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Abstract

We used cDNA microarray gene expression profiling to characterize the transcriptional response to exposure of cultured mouse cerebral cortical neurons to hypoxia for 24 hr. Of 11,200 genes examined, 1,405 (12.5%) were induced or repressed at least 1.5-fold, whereas 26 known genes were induced and 20 known genes were repressed at least 2.5-fold. The most strongly induced genes included genes coding for endoplasmic reticulum proteins (Ero1L/Giig11, Sac1p, Ddit3/Gadd153), proteins involved in ubiquitination (Arih2, P4hb), proteins induced by hypoxia in non-neuronal systems (Gpi1, Aldo1, Anxa2, Hig1), and proteins that might promote cell death (Gas5, Egr1, Ndr1, Vdac2). These findings reinforce the importance of endoplasmic reticulum-based mechanisms and of protein-ubiquitination pathways in the neuronal response to hypoxia.

Original languageEnglish
Article number454310
Pages (from-to)1105-1112
Number of pages8
JournalNeurochemical Research
Volume27
Issue number10
DOIs
StatePublished - 1 Jan 2002

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Microarray Analysis
Microarrays
Oligonucleotide Array Sequence Analysis
Gene expression
Complementary DNA
Genes
Gene Expression
Ubiquitination
Proteins
Endoplasmic Reticulum
Gene Expression Profiling
Cell death
Neurons
Cell Death
In Vitro Techniques
Hypoxia

Keywords

  • Endoplasmic reticulum
  • Hypoxia
  • Hypoxia-inducible factor-1
  • Microarray
  • Ubiquitination

Cite this

Jin, K., Mao, X. O., Eshoo, M. W., Del Rio, G., Rao, R., Chen, D., ... Greenberg, D. A. (2002). cDNA microarray analysis of changes in gene expression induced by neuronal hypoxia in vitro. Neurochemical Research, 27(10), 1105-1112. [454310]. https://doi.org/10.1023/A:1020913123054
Jin, K. ; Mao, X. O. ; Eshoo, M. W. ; Del Rio, G. ; Rao, R. ; Chen, D. ; Simon, R. P. ; Greenberg, D. A. / cDNA microarray analysis of changes in gene expression induced by neuronal hypoxia in vitro. In: Neurochemical Research. 2002 ; Vol. 27, No. 10. pp. 1105-1112.
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Jin, K, Mao, XO, Eshoo, MW, Del Rio, G, Rao, R, Chen, D, Simon, RP & Greenberg, DA 2002, 'cDNA microarray analysis of changes in gene expression induced by neuronal hypoxia in vitro', Neurochemical Research, vol. 27, no. 10, 454310, pp. 1105-1112. https://doi.org/10.1023/A:1020913123054

cDNA microarray analysis of changes in gene expression induced by neuronal hypoxia in vitro. / Jin, K.; Mao, X. O.; Eshoo, M. W.; Del Rio, G.; Rao, R.; Chen, D.; Simon, R. P.; Greenberg, D. A.

In: Neurochemical Research, Vol. 27, No. 10, 454310, 01.01.2002, p. 1105-1112.

Research output: Contribution to journalArticle

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