TY - JOUR
T1 - cDNA microarray analysis of changes in gene expression induced by neuronal hypoxia in vitro
AU - Jin, K.
AU - Mao, X. O.
AU - Eshoo, M. W.
AU - Del Rio, G.
AU - Rao, R.
AU - Chen, D.
AU - Simon, R. P.
AU - Greenberg, D. A.
PY - 2002
Y1 - 2002
N2 - We used cDNA microarray gene expression profiling to characterize the transcriptional response to exposure of cultured mouse cerebral cortical neurons to hypoxia for 24 hr. Of 11,200 genes examined, 1,405 (12.5%) were induced or repressed at least 1.5-fold, whereas 26 known genes were induced and 20 known genes were repressed at least 2.5-fold. The most strongly induced genes included genes coding for endoplasmic reticulum proteins (Ero1L/Giig11, Sac1p, Ddit3/Gadd153), proteins involved in ubiquitination (Arih2, P4hb), proteins induced by hypoxia in non-neuronal systems (Gpi1, Aldo1, Anxa2, Hig1), and proteins that might promote cell death (Gas5, Egr1, Ndr1, Vdac2). These findings reinforce the importance of endoplasmic reticulum-based mechanisms and of protein-ubiquitination pathways in the neuronal response to hypoxia.
AB - We used cDNA microarray gene expression profiling to characterize the transcriptional response to exposure of cultured mouse cerebral cortical neurons to hypoxia for 24 hr. Of 11,200 genes examined, 1,405 (12.5%) were induced or repressed at least 1.5-fold, whereas 26 known genes were induced and 20 known genes were repressed at least 2.5-fold. The most strongly induced genes included genes coding for endoplasmic reticulum proteins (Ero1L/Giig11, Sac1p, Ddit3/Gadd153), proteins involved in ubiquitination (Arih2, P4hb), proteins induced by hypoxia in non-neuronal systems (Gpi1, Aldo1, Anxa2, Hig1), and proteins that might promote cell death (Gas5, Egr1, Ndr1, Vdac2). These findings reinforce the importance of endoplasmic reticulum-based mechanisms and of protein-ubiquitination pathways in the neuronal response to hypoxia.
KW - Endoplasmic reticulum
KW - Hypoxia
KW - Hypoxia-inducible factor-1
KW - Microarray
KW - Ubiquitination
UR - http://www.scopus.com/inward/record.url?scp=0036807952&partnerID=8YFLogxK
U2 - 10.1023/A:1020913123054
DO - 10.1023/A:1020913123054
M3 - Article
C2 - 12462408
AN - SCOPUS:0036807952
SN - 0364-3190
VL - 27
SP - 1105
EP - 1112
JO - Neurochemical Research
JF - Neurochemical Research
IS - 10
M1 - 454310
ER -