cDNA microarray analysis of changes in gene expression induced by neuronal hypoxia in vitro

K. Jin, X. O. Mao, M. W. Eshoo, G. Del Rio, R. Rao, D. Chen, R. P. Simon, D. A. Greenberg

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Abstract

We used cDNA microarray gene expression profiling to characterize the transcriptional response to exposure of cultured mouse cerebral cortical neurons to hypoxia for 24 hr. Of 11,200 genes examined, 1,405 (12.5%) were induced or repressed at least 1.5-fold, whereas 26 known genes were induced and 20 known genes were repressed at least 2.5-fold. The most strongly induced genes included genes coding for endoplasmic reticulum proteins (Ero1L/Giig11, Sac1p, Ddit3/Gadd153), proteins involved in ubiquitination (Arih2, P4hb), proteins induced by hypoxia in non-neuronal systems (Gpi1, Aldo1, Anxa2, Hig1), and proteins that might promote cell death (Gas5, Egr1, Ndr1, Vdac2). These findings reinforce the importance of endoplasmic reticulum-based mechanisms and of protein-ubiquitination pathways in the neuronal response to hypoxia.

Original languageEnglish
Article number454310
Pages (from-to)1105-1112
Number of pages8
JournalNeurochemical Research
Volume27
Issue number10
DOIs
StatePublished - 1 Jan 2002

Keywords

  • Endoplasmic reticulum
  • Hypoxia
  • Hypoxia-inducible factor-1
  • Microarray
  • Ubiquitination

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    Jin, K., Mao, X. O., Eshoo, M. W., Del Rio, G., Rao, R., Chen, D., Simon, R. P., & Greenberg, D. A. (2002). cDNA microarray analysis of changes in gene expression induced by neuronal hypoxia in vitro. Neurochemical Research, 27(10), 1105-1112. [454310]. https://doi.org/10.1023/A:1020913123054