CDNA cloning and sequence analysis of bubaline growth hormone

Sulekha Verma, Anuja Ghorpade, Gunjan Tiwari, P. Das, Lalit C. Garg

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

The cDNA for Bubalus bubalis growth hormone (GH) has been cloned and sequence determined through RT-PCR approach. The nucleotide sequence of bubaline GH cDNA was in a single reading frame coding for a protein of 191 residues comprising a putative signal sequence of 27 amino acids. Homol-ogy comparison of the sequence with other mammalian GH cDNAs showed a very high degree of evolutionary conservation. Bubaline GH sequence shared a homology of 99.5%, 99.5%, 98.6%, 87.6% and 61.9% with that of ovine, caprine, bovine, porcine and human, respectively at amino acid level.

Original languageEnglish
Pages (from-to)101-103
Number of pages3
JournalMitochondrial DNA
Volume10
Issue number2
DOIs
StatePublished - 1999

Keywords

  • Growth hormone
  • PCR
  • Recombinant DNA
  • cDNA cloning

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