CDNA cloning and sequence analysis of bubaline growth hormone

Sulekha Verma; Anuja Ghorpade; Gunjan Tiwari; P. Das; Lalit C. Garg

doi:10.3109/10425179909008425

CDNA cloning and sequence analysis of bubaline growth hormone

Sulekha Verma, Anuja Ghorpade, Gunjan Tiwari, P. Das, Lalit C. Garg

School of Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

The cDNA for Bubalus bubalis growth hormone (GH) has been cloned and sequence determined through RT-PCR approach. The nucleotide sequence of bubaline GH cDNA was in a single reading frame coding for a protein of 191 residues comprising a putative signal sequence of 27 amino acids. Homol-ogy comparison of the sequence with other mammalian GH cDNAs showed a very high degree of evolutionary conservation. Bubaline GH sequence shared a homology of 99.5%, 99.5%, 98.6%, 87.6% and 61.9% with that of ovine, caprine, bovine, porcine and human, respectively at amino acid level.

Original language	English
Pages (from-to)	101-103
Number of pages	3
Journal	Mitochondrial DNA
Volume	10
Issue number	2
DOIs	https://doi.org/10.3109/10425179909008425
State	Published - 1999

Keywords

Growth hormone
PCR
Recombinant DNA
cDNA cloning

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title = "CDNA cloning and sequence analysis of bubaline growth hormone",

abstract = "The cDNA for Bubalus bubalis growth hormone (GH) has been cloned and sequence determined through RT-PCR approach. The nucleotide sequence of bubaline GH cDNA was in a single reading frame coding for a protein of 191 residues comprising a putative signal sequence of 27 amino acids. Homol-ogy comparison of the sequence with other mammalian GH cDNAs showed a very high degree of evolutionary conservation. Bubaline GH sequence shared a homology of 99.5%, 99.5%, 98.6%, 87.6% and 61.9% with that of ovine, caprine, bovine, porcine and human, respectively at amino acid level.",

keywords = "Growth hormone, PCR, Recombinant DNA, cDNA cloning",

author = "Sulekha Verma and Anuja Ghorpade and Gunjan Tiwari and P. Das and Garg, {Lalit C.}",

note = "Funding Information: This work was supported by a grant from the Dept. of Biotechnology, Govt. of India and Senior Research Associateship to P. D. by CSIR is acknowledged.",

year = "1999",

doi = "10.3109/10425179909008425",

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T1 - CDNA cloning and sequence analysis of bubaline growth hormone

AU - Verma, Sulekha

AU - Ghorpade, Anuja

AU - Tiwari, Gunjan

AU - Das, P.

AU - Garg, Lalit C.

N1 - Funding Information: This work was supported by a grant from the Dept. of Biotechnology, Govt. of India and Senior Research Associateship to P. D. by CSIR is acknowledged.

PY - 1999

Y1 - 1999

N2 - The cDNA for Bubalus bubalis growth hormone (GH) has been cloned and sequence determined through RT-PCR approach. The nucleotide sequence of bubaline GH cDNA was in a single reading frame coding for a protein of 191 residues comprising a putative signal sequence of 27 amino acids. Homol-ogy comparison of the sequence with other mammalian GH cDNAs showed a very high degree of evolutionary conservation. Bubaline GH sequence shared a homology of 99.5%, 99.5%, 98.6%, 87.6% and 61.9% with that of ovine, caprine, bovine, porcine and human, respectively at amino acid level.

AB - The cDNA for Bubalus bubalis growth hormone (GH) has been cloned and sequence determined through RT-PCR approach. The nucleotide sequence of bubaline GH cDNA was in a single reading frame coding for a protein of 191 residues comprising a putative signal sequence of 27 amino acids. Homol-ogy comparison of the sequence with other mammalian GH cDNAs showed a very high degree of evolutionary conservation. Bubaline GH sequence shared a homology of 99.5%, 99.5%, 98.6%, 87.6% and 61.9% with that of ovine, caprine, bovine, porcine and human, respectively at amino acid level.

KW - Growth hormone

KW - PCR

KW - Recombinant DNA

KW - cDNA cloning

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DO - 10.3109/10425179909008425

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SN - 1940-1736

VL - 10

SP - 101

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JO - Mitochondrial DNA

JF - Mitochondrial DNA

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ER -

CDNA cloning and sequence analysis of bubaline growth hormone

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