Abstract
The cDNA for Bubalus bubalis growth hormone (GH) has been cloned and sequence determined through RT-PCR approach. The nucleotide sequence of bubaline GH cDNA was in a single reading frame coding for a protein of 191 residues comprising a putative signal sequence of 27 amino acids. Homol-ogy comparison of the sequence with other mammalian GH cDNAs showed a very high degree of evolutionary conservation. Bubaline GH sequence shared a homology of 99.5%, 99.5%, 98.6%, 87.6% and 61.9% with that of ovine, caprine, bovine, porcine and human, respectively at amino acid level.
Original language | English |
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Pages (from-to) | 101-103 |
Number of pages | 3 |
Journal | Mitochondrial DNA |
Volume | 10 |
Issue number | 2 |
DOIs | |
State | Published - 1999 |
Keywords
- Growth hormone
- PCR
- Recombinant DNA
- cDNA cloning