Carbon nanotube-based biosensors

Roberto Ramoni, Maria Staiano, Stefano Bellucci, Ignacy Grycznyski, Zygmunt Grycznyski, Roberta Crescenzo, Luisa Iozzino, Shashank Bharill, Virna Conti, Stefano Grolli, Sabato D'Auria

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

An easy and rapid detection of hazardous compounds is crucial for making on-the-spot irreversible decisions at airport security gates, luggage storage rooms, and other crowded public places, such as stadia, concert halls, etc. In the present study we carried out a preliminary investigation into the possibility of utilizing as advanced nano-biosensors a mutant form of the bovine odorant-binding protein (bOBP) immobilized onto carbon nanotubes. In particular, after immobilization of the protein on the carbon nanotubes we developed a competitive resonance energy transfer (RET) assay between the protein tryptophan residues located at the positions 17 and 133 (W17 and W133) and the 1-amino-anthracene (AMA), a molecule that fits in the binding site of bOBP. The bOBP-AMA complex emitted light in the visible region upon excitation of the Trp donors. However, the addition of an odorant molecule to the bOBP-AMA complex displaced AMA from the binding site making the carbon nanotubes colorless. The results presented in this work are very promising for the realization of a color on/ color off b-OBP-based biosensor for the initial indication of hazardous compounds in the environment.

Original languageEnglish
Article number474201
JournalJournal of Physics Condensed Matter
Volume20
Issue number47
DOIs
StatePublished - 6 Nov 2008

Fingerprint Dive into the research topics of 'Carbon nanotube-based biosensors'. Together they form a unique fingerprint.

  • Cite this

    Ramoni, R., Staiano, M., Bellucci, S., Grycznyski, I., Grycznyski, Z., Crescenzo, R., Iozzino, L., Bharill, S., Conti, V., Grolli, S., & D'Auria, S. (2008). Carbon nanotube-based biosensors. Journal of Physics Condensed Matter, 20(47), [474201]. https://doi.org/10.1088/0953-8984/20/47/474201