TY - JOUR
T1 - Carbon nanotube-based biosensors
AU - Ramoni, Roberto
AU - Staiano, Maria
AU - Bellucci, Stefano
AU - Grycznyski, Ignacy
AU - Grycznyski, Zygmunt
AU - Crescenzo, Roberta
AU - Iozzino, Luisa
AU - Bharill, Shashank
AU - Conti, Virna
AU - Grolli, Stefano
AU - D'Auria, Sabato
N1 - Funding Information:
N. Yang and Dr. Chen contributed equally to this work. The research is co-supported by National Natural Science Foundation of China (Project no. 51303033), Guangxi Natural Science Foundation (Grant no. 2011GXNSFD018027 ), China Postdoctoral Science Foundation (Project no. 2014M550065), Guangxi Experiment Center of Information Science (Project no. 20130107) and Guangxi's Key Laboratory Foundation of Manufacturing Systems and Advanced Manufacturing Technology (Project no. 13-051-09-003Z).
PY - 2008/11/6
Y1 - 2008/11/6
N2 - An easy and rapid detection of hazardous compounds is crucial for making on-the-spot irreversible decisions at airport security gates, luggage storage rooms, and other crowded public places, such as stadia, concert halls, etc. In the present study we carried out a preliminary investigation into the possibility of utilizing as advanced nano-biosensors a mutant form of the bovine odorant-binding protein (bOBP) immobilized onto carbon nanotubes. In particular, after immobilization of the protein on the carbon nanotubes we developed a competitive resonance energy transfer (RET) assay between the protein tryptophan residues located at the positions 17 and 133 (W17 and W133) and the 1-amino-anthracene (AMA), a molecule that fits in the binding site of bOBP. The bOBP-AMA complex emitted light in the visible region upon excitation of the Trp donors. However, the addition of an odorant molecule to the bOBP-AMA complex displaced AMA from the binding site making the carbon nanotubes colorless. The results presented in this work are very promising for the realization of a color on/ color off b-OBP-based biosensor for the initial indication of hazardous compounds in the environment.
AB - An easy and rapid detection of hazardous compounds is crucial for making on-the-spot irreversible decisions at airport security gates, luggage storage rooms, and other crowded public places, such as stadia, concert halls, etc. In the present study we carried out a preliminary investigation into the possibility of utilizing as advanced nano-biosensors a mutant form of the bovine odorant-binding protein (bOBP) immobilized onto carbon nanotubes. In particular, after immobilization of the protein on the carbon nanotubes we developed a competitive resonance energy transfer (RET) assay between the protein tryptophan residues located at the positions 17 and 133 (W17 and W133) and the 1-amino-anthracene (AMA), a molecule that fits in the binding site of bOBP. The bOBP-AMA complex emitted light in the visible region upon excitation of the Trp donors. However, the addition of an odorant molecule to the bOBP-AMA complex displaced AMA from the binding site making the carbon nanotubes colorless. The results presented in this work are very promising for the realization of a color on/ color off b-OBP-based biosensor for the initial indication of hazardous compounds in the environment.
UR - http://www.scopus.com/inward/record.url?scp=58149302546&partnerID=8YFLogxK
U2 - 10.1088/0953-8984/20/47/474201
DO - 10.1088/0953-8984/20/47/474201
M3 - Article
AN - SCOPUS:58149302546
SN - 0953-8984
VL - 20
JO - Journal of Physics Condensed Matter
JF - Journal of Physics Condensed Matter
IS - 47
M1 - 474201
ER -