Carbon nanotube-based biosensors

Roberto Ramoni, Maria Staiano, Stefano Bellucci, Ignacy Grycznyski, Zygmunt Gryczynski, Roberta Crescenzo, Luisa Iozzino, Shashank Bharill, Virna Conti, Stefano Grolli, Sabato D'Auria

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

An easy and rapid detection of hazardous compounds is crucial for making on-the-spot irreversible decisions at airport security gates, luggage storage rooms, and other crowded public places, such as stadia, concert halls, etc. In the present study we carried out a preliminary investigation into the possibility of utilizing as advanced nano-biosensors a mutant form of the bovine odorant-binding protein (bOBP) immobilized onto carbon nanotubes. In particular, after immobilization of the protein on the carbon nanotubes we developed a competitive resonance energy transfer (RET) assay between the protein tryptophan residues located at the positions 17 and 133 (W17 and W133) and the 1-amino-anthracene (AMA), a molecule that fits in the binding site of bOBP. The bOBP-AMA complex emitted light in the visible region upon excitation of the Trp donors. However, the addition of an odorant molecule to the bOBP-AMA complex displaced AMA from the binding site making the carbon nanotubes colorless. The results presented in this work are very promising for the realization of a color on/ color off b-OBP-based biosensor for the initial indication of hazardous compounds in the environment.

Original languageEnglish
Article number474201
JournalJournal of Physics Condensed Matter
Volume20
Issue number47
DOIs
StatePublished - 6 Nov 2008

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Carbon Nanotubes
Anthracene
bioinstrumentation
Biosensors
Carbon nanotubes
carbon nanotubes
anthracene
proteins
Binding sites
Airport security
Binding Sites
Color
Proteins
Stadiums
Molecules
airport security
Tryptophan
Energy transfer
color
Assays

Cite this

Ramoni, R., Staiano, M., Bellucci, S., Grycznyski, I., Gryczynski, Z., Crescenzo, R., ... D'Auria, S. (2008). Carbon nanotube-based biosensors. Journal of Physics Condensed Matter, 20(47), [474201]. https://doi.org/10.1088/0953-8984/20/47/474201
Ramoni, Roberto ; Staiano, Maria ; Bellucci, Stefano ; Grycznyski, Ignacy ; Gryczynski, Zygmunt ; Crescenzo, Roberta ; Iozzino, Luisa ; Bharill, Shashank ; Conti, Virna ; Grolli, Stefano ; D'Auria, Sabato. / Carbon nanotube-based biosensors. In: Journal of Physics Condensed Matter. 2008 ; Vol. 20, No. 47.
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Ramoni, R, Staiano, M, Bellucci, S, Grycznyski, I, Gryczynski, Z, Crescenzo, R, Iozzino, L, Bharill, S, Conti, V, Grolli, S & D'Auria, S 2008, 'Carbon nanotube-based biosensors', Journal of Physics Condensed Matter, vol. 20, no. 47, 474201. https://doi.org/10.1088/0953-8984/20/47/474201

Carbon nanotube-based biosensors. / Ramoni, Roberto; Staiano, Maria; Bellucci, Stefano; Grycznyski, Ignacy; Gryczynski, Zygmunt; Crescenzo, Roberta; Iozzino, Luisa; Bharill, Shashank; Conti, Virna; Grolli, Stefano; D'Auria, Sabato.

In: Journal of Physics Condensed Matter, Vol. 20, No. 47, 474201, 06.11.2008.

Research output: Contribution to journalArticle

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Ramoni R, Staiano M, Bellucci S, Grycznyski I, Gryczynski Z, Crescenzo R et al. Carbon nanotube-based biosensors. Journal of Physics Condensed Matter. 2008 Nov 6;20(47). 474201. https://doi.org/10.1088/0953-8984/20/47/474201