Capillary electrophoresis of MiniSTR markers to genotype highly degraded DNA samples

Research output: Chapter in Book/Report/Conference proceedingChapter

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Abstract

The amplification of short tandem repeat (STR) markers throughout the human nuclear DNA genome are used to associate crime scene evidence to the perpetrator's profile in criminal investigations. For highly challenged or compromised materials such as stains exposed to the elements, skeletal remains from missing persons cases, or fragmented and degraded samples from mass disasters, obtaining a full STR profile may be difficult if not impossible. With the introduction of short amplicon STR or "miniSTR" typing, it is possible to obtain STR genetic information from highly challenged samples without the need to sequence the hypervariable regions of the mitochondrial DNA (mtDNA) genome. Non-Combined DNA Index System (CODIS) STR markers have been developed to obtain information beyond the core CODIS loci. This chapter will focus on the steps necessary to prepare and use one of the non-CODIS (NC) multiplexes, NC01 (Coble and Butler 2005), for analysis on capillary electrophoresis instrumentation.

Original languageEnglish
Title of host publicationDNA Electrophoresis Protocols for Forensic Genetics
EditorsAntonio Alonso
Pages31-42
Number of pages12
DOIs
Publication statusPublished - 20 Jan 2012

Publication series

NameMethods in Molecular Biology
Volume830
ISSN (Print)1064-3745

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Keywords

  • DNA typing
  • Degraded DNA
  • Mini-STRs
  • PCR
  • STR

Cite this

Coble, M. D. (2012). Capillary electrophoresis of MiniSTR markers to genotype highly degraded DNA samples. In A. Alonso (Ed.), DNA Electrophoresis Protocols for Forensic Genetics (pp. 31-42). (Methods in Molecular Biology; Vol. 830). https://doi.org/10.1007/978-1-61779-461-2_3