Canonical transient receptor potential 1 channel is involved in contractile function of glomerular mesangial cells

Juan Du, Sherry Sours-Brothers, Rashadd Coleman, Min Ding, Sarabeth Graham, De Hu Kong, Rong Ma

Research output: Contribution to journalArticle

53 Scopus citations

Abstract

Contractility of mesangial cells (MC) is tightly controlled by [Ca 2+]i. Ca2+ influx across the plasma membrane constitutes a major component of mesangial responses to vasoconstrictors. Canonical transient receptor potential 1 (TRPC1) is a Ca2+-permeable cation channel in a variety of cell types. This study was performed to investigate whether TRPC1 takes part in vasoconstrictor-induced mesangial contraction by mediating Ca2+ entry. It was found that angiotensin II (AngII) evoked remarkable contraction of the cultured MC. Downregulation of TRPC1 using RNA interference significantly attenuated the contractile response. Infusion of AngII or endothelin-1 in rats caused a decrease in GFR. The GFR decline was significantly reduced by infusion of TRPC1 antibody that targets an extracellular domain in the pore region of TRPC1 channel. However, the treatment of TRPC1 antibody did not affect the AngII-induced vasopressing effect. Electrophysiologic experiments revealed that functional or biologic inhibition of TRPC1 significantly depressed AngII-induced channel activation. Fura-2 fluorescence-indicated that Ca2+ entry in response to AngII stimulation was also dramatically inhibited by TRPC1 antibody and TRPC1-specific RNA interference. These results suggest that TRPC1 plays an important role in controlling contractile function of MC. Mediation of Ca2+ entry might be the underlying mechanism for the TRPC1-associated MC contraction.

Original languageEnglish
Pages (from-to)1437-1445
Number of pages9
JournalJournal of the American Society of Nephrology
Volume18
Issue number5
DOIs
StatePublished - 1 May 2007

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