C-MYC controlled TIP110 protein expression regulates OCT4 mRNA splicing in human embryonic stem cells

Ying Liu, Khalid A. Timani, Xuan Ou, Hal E. Broxmeyer, Johnny Jianglin He

Research output: Contribution to journalArticleResearchpeer-review

17 Citations (Scopus)

Abstract

We reported previously that HIV-1 the Tat-interacting protein of 110 kDa (TIP110; P110(NRB)/SART3/p110) is important in regulation of hematopoiesis, and in maintaining pluripotent factor (NANOG, OCT4, and SOX2) expression in and pluripotency of human embryonic stem cells (hESCs). Here we show that TIP110 expression in hESCs line H9 and embryonal carcinoma cell line NT-2 is regulated by C-MYC expression in ESCs through an E box present in the TIP110 promoter region. Through up- and down- modulation of expression, TIP110 induces OCT4 exon 1a inclusion and exon 1b skipping in our OCT4 minigene model. Thus, TIP110 expression in ESCs regulates alternative splicing of OCT4, an event required for pluripotency of ESCs.

Original languageEnglish
Pages (from-to)689-694
Number of pages6
JournalStem Cells and Development
Volume22
Issue number5
DOIs
StatePublished - 1 Mar 2013

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Exons
tat Gene Products
Embryonal Carcinoma Stem Cells
Cell Line
Messenger RNA
Hematopoiesis
Alternative Splicing
Genetic Promoter Regions
HIV-1
Proteins
Human Embryonic Stem Cells
methyl(arginyl)-lysyl-prolyl-tryptophyl-tert-leucyl-leucine

Cite this

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title = "C-MYC controlled TIP110 protein expression regulates OCT4 mRNA splicing in human embryonic stem cells",
abstract = "We reported previously that HIV-1 the Tat-interacting protein of 110 kDa (TIP110; P110(NRB)/SART3/p110) is important in regulation of hematopoiesis, and in maintaining pluripotent factor (NANOG, OCT4, and SOX2) expression in and pluripotency of human embryonic stem cells (hESCs). Here we show that TIP110 expression in hESCs line H9 and embryonal carcinoma cell line NT-2 is regulated by C-MYC expression in ESCs through an E box present in the TIP110 promoter region. Through up- and down- modulation of expression, TIP110 induces OCT4 exon 1a inclusion and exon 1b skipping in our OCT4 minigene model. Thus, TIP110 expression in ESCs regulates alternative splicing of OCT4, an event required for pluripotency of ESCs.",
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C-MYC controlled TIP110 protein expression regulates OCT4 mRNA splicing in human embryonic stem cells. / Liu, Ying; Timani, Khalid A.; Ou, Xuan; Broxmeyer, Hal E.; He, Johnny Jianglin.

In: Stem Cells and Development, Vol. 22, No. 5, 01.03.2013, p. 689-694.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - C-MYC controlled TIP110 protein expression regulates OCT4 mRNA splicing in human embryonic stem cells

AU - Liu, Ying

AU - Timani, Khalid A.

AU - Ou, Xuan

AU - Broxmeyer, Hal E.

AU - He, Johnny Jianglin

PY - 2013/3/1

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AB - We reported previously that HIV-1 the Tat-interacting protein of 110 kDa (TIP110; P110(NRB)/SART3/p110) is important in regulation of hematopoiesis, and in maintaining pluripotent factor (NANOG, OCT4, and SOX2) expression in and pluripotency of human embryonic stem cells (hESCs). Here we show that TIP110 expression in hESCs line H9 and embryonal carcinoma cell line NT-2 is regulated by C-MYC expression in ESCs through an E box present in the TIP110 promoter region. Through up- and down- modulation of expression, TIP110 induces OCT4 exon 1a inclusion and exon 1b skipping in our OCT4 minigene model. Thus, TIP110 expression in ESCs regulates alternative splicing of OCT4, an event required for pluripotency of ESCs.

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