BSA AU clusters as a probe for enhanced fluorescence detection using multipulse excitation scheme

Research output: Contribution to journalArticleResearchpeer-review

1 Citation (Scopus)

Abstract

Although BSA Au clusters fluoresce in red region (λmax: 650nm), they are of limited use due to low fluorescence quantum yield (~6%). Here we report an enhanced fluorescence imaging application of fluorescent bio-nano probe BSA Au clusters using multipulse excitation scheme. Multipulse excitation takes advantage of long fluorescence lifetime (> 1μs) of BSA Au clusters and enhances its fluorescence intensity 15 times over short lived cellular auto-fluorescence. Moreover we have also shown that by using time gated detection strategy signal (fluorescence of BSA Au clusters) to noise (auto-fluorescence) ratio can be increased by 30 fold. Thereby with multipulse excitation long lifetime probes can be used to develop biochemical assays and perform optical imaging with zero background.

Original languageEnglish
Pages (from-to)1139-1144
Number of pages6
JournalCurrent Pharmaceutical Biotechnology
Volume14
Issue number13
DOIs
StatePublished - 1 Jan 2014

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Fluorescence
Optical Imaging
Noise

Keywords

  • Autofluorescence
  • BSA au clusters
  • Long lifetime
  • Multipulse excitation
  • Signal to noise

Cite this

@article{18befe8126c34d99aa6ce1759fd8081e,
title = "BSA AU clusters as a probe for enhanced fluorescence detection using multipulse excitation scheme",
abstract = "Although BSA Au clusters fluoresce in red region (λmax: 650nm), they are of limited use due to low fluorescence quantum yield (~6{\%}). Here we report an enhanced fluorescence imaging application of fluorescent bio-nano probe BSA Au clusters using multipulse excitation scheme. Multipulse excitation takes advantage of long fluorescence lifetime (> 1μs) of BSA Au clusters and enhances its fluorescence intensity 15 times over short lived cellular auto-fluorescence. Moreover we have also shown that by using time gated detection strategy signal (fluorescence of BSA Au clusters) to noise (auto-fluorescence) ratio can be increased by 30 fold. Thereby with multipulse excitation long lifetime probes can be used to develop biochemical assays and perform optical imaging with zero background.",
keywords = "Autofluorescence, BSA au clusters, Long lifetime, Multipulse excitation, Signal to noise",
author = "Raut, {Sangram Limbaji} and Ryan Rich and Rafal Fudala and R. Kokate and Kimball, {J. D.} and Julian Borejdo and Vishwanatha, {Jamboor K.} and Zygmunt Gryczynski and Ignacy Gryczynski",
year = "2014",
month = "1",
day = "1",
doi = "10.2174/1389201015666140523161038",
language = "English",
volume = "14",
pages = "1139--1144",
journal = "Current Pharmaceutical Biotechnology",
issn = "1389-2010",
publisher = "Bentham Science Publishers B.V.",
number = "13",

}

BSA AU clusters as a probe for enhanced fluorescence detection using multipulse excitation scheme. / Raut, Sangram Limbaji; Rich, Ryan; Fudala, Rafal; Kokate, R.; Kimball, J. D.; Borejdo, Julian; Vishwanatha, Jamboor K.; Gryczynski, Zygmunt; Gryczynski, Ignacy.

In: Current Pharmaceutical Biotechnology, Vol. 14, No. 13, 01.01.2014, p. 1139-1144.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - BSA AU clusters as a probe for enhanced fluorescence detection using multipulse excitation scheme

AU - Raut, Sangram Limbaji

AU - Rich, Ryan

AU - Fudala, Rafal

AU - Kokate, R.

AU - Kimball, J. D.

AU - Borejdo, Julian

AU - Vishwanatha, Jamboor K.

AU - Gryczynski, Zygmunt

AU - Gryczynski, Ignacy

PY - 2014/1/1

Y1 - 2014/1/1

N2 - Although BSA Au clusters fluoresce in red region (λmax: 650nm), they are of limited use due to low fluorescence quantum yield (~6%). Here we report an enhanced fluorescence imaging application of fluorescent bio-nano probe BSA Au clusters using multipulse excitation scheme. Multipulse excitation takes advantage of long fluorescence lifetime (> 1μs) of BSA Au clusters and enhances its fluorescence intensity 15 times over short lived cellular auto-fluorescence. Moreover we have also shown that by using time gated detection strategy signal (fluorescence of BSA Au clusters) to noise (auto-fluorescence) ratio can be increased by 30 fold. Thereby with multipulse excitation long lifetime probes can be used to develop biochemical assays and perform optical imaging with zero background.

AB - Although BSA Au clusters fluoresce in red region (λmax: 650nm), they are of limited use due to low fluorescence quantum yield (~6%). Here we report an enhanced fluorescence imaging application of fluorescent bio-nano probe BSA Au clusters using multipulse excitation scheme. Multipulse excitation takes advantage of long fluorescence lifetime (> 1μs) of BSA Au clusters and enhances its fluorescence intensity 15 times over short lived cellular auto-fluorescence. Moreover we have also shown that by using time gated detection strategy signal (fluorescence of BSA Au clusters) to noise (auto-fluorescence) ratio can be increased by 30 fold. Thereby with multipulse excitation long lifetime probes can be used to develop biochemical assays and perform optical imaging with zero background.

KW - Autofluorescence

KW - BSA au clusters

KW - Long lifetime

KW - Multipulse excitation

KW - Signal to noise

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JO - Current Pharmaceutical Biotechnology

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