TY - JOUR
T1 - Blue-light-controlled photoprotection in plants at the level of the photosynthetic antenna complex LHCII
AU - Gruszecki, Wieslaw I.
AU - Luchowski, Rafal
AU - Zubik, Monika
AU - Grudzinski, Wojciech
AU - Janik, Ewa
AU - Gospodarek, Malgorzata
AU - Goc, Jacek
AU - Gryczynski, Zygmunt
AU - Gryczynski, Ignacy
N1 - Funding Information:
This research was financed by the Ministry of Science and Higher Education of Poland from the funds for science in the years 2008–2011 within the research project no. N303 285034. R.L. acknowledges the post-doctoral fellowship from the Ministry of Science and Higher Education of Poland ( Grant no. 17/MOB/2007/0 ).
PY - 2010/1/1
Y1 - 2010/1/1
N2 - Plants have developed several adaptive regulatory mechanisms, operating at all the organization levels, to optimize utilization of light energy and to protect themselves against over-excitation-related damage. We report activity of a previously unknown possible regulatory mechanism that operates at the molecular level of the major photosynthetic pigment-protein complexes of plants, LHCII. This mechanism is driven exclusively by blue light, operates in the trimeric but not in the monomeric complex, and results in singlet excitation quenching leading to thermal energy dissipation. The conclusions are based on single molecule fluorescence lifetime analysis, direct measurements of thermal energy dissipation by photo-thermal spectroscopy, and on fluorescence spectroscopy. Possible molecular mechanisms involved in the blue-light-induced photoprotective effect are discussed, including xanthophyll photo-isomerization and the thermo-optic effect.
AB - Plants have developed several adaptive regulatory mechanisms, operating at all the organization levels, to optimize utilization of light energy and to protect themselves against over-excitation-related damage. We report activity of a previously unknown possible regulatory mechanism that operates at the molecular level of the major photosynthetic pigment-protein complexes of plants, LHCII. This mechanism is driven exclusively by blue light, operates in the trimeric but not in the monomeric complex, and results in singlet excitation quenching leading to thermal energy dissipation. The conclusions are based on single molecule fluorescence lifetime analysis, direct measurements of thermal energy dissipation by photo-thermal spectroscopy, and on fluorescence spectroscopy. Possible molecular mechanisms involved in the blue-light-induced photoprotective effect are discussed, including xanthophyll photo-isomerization and the thermo-optic effect.
KW - Blue-light effect
KW - FLIM
KW - LHCII
KW - Photoprotection
KW - Single molecule spectroscopy
UR - http://www.scopus.com/inward/record.url?scp=70450224888&partnerID=8YFLogxK
U2 - 10.1016/j.jplph.2009.07.012
DO - 10.1016/j.jplph.2009.07.012
M3 - Article
C2 - 19699007
AN - SCOPUS:70450224888
SN - 0176-1617
VL - 167
SP - 69
EP - 73
JO - Journal of Plant Physiology
JF - Journal of Plant Physiology
IS - 1
ER -