TY - JOUR
T1 - Blood-based inflammation biomarkers of neurocognitive impairment in people living with HIV
AU - Swanta, Naomi
AU - Aryal, Subhash
AU - Nejtek, Vicki
AU - Shenoy, Sangeeta
AU - Ghorpade, Anuja
AU - Borgmann, Kathleen
N1 - Funding Information:
We thank the following for their kind assistance with participant recruitment and medical history reviews. Dr.?Barbara Atkinson?at the Infectious Disease Clinic at UNTHSC and her nurse Ms. Cindy Bishop. Dr. Catherine Colquitt at Tarrant County Public Health Preventive Medicine Clinic, Fort Worth, Texas and her support staff Ms. Deborah Radaford, Ms. Marry Ellen Yarrish, and Ms. Blanca Delatorre. Ms. Victoria Langston at the John Peter Smith (JPS) Healing Wings HIV/AIDS Center, Fort Worth, Texas. All the staff and clinicians at the AIDS Health Care Foundation, Fort Worth, Texas, the AIDS Outreach Center, Fort Worth, Texas, Samaritan House, Fort Worth, Texas, and Tarrant County Infectious Diseases Associates, Fort Worth, Texas. We appreciate the effort of our other study staff. Ms. Satomi Stacy, who served as a research coordinator who collected and processed patient samples. Dr. Manmeet Kaur Mamik, Ms. Lin Tang and the late Dr. Brian Molles for processing patient samples. Several translators facilitated communication with our White Hispanic populations, we thank Ms. Monica Castillo, Ms. Haydee Izurieta Munoz, Mr. Raul Vintimilla, Ms. Adriana Gamboa Guzman and Dr. Ricardo Belmares. Several medical research assistants assisted with data entry and scheduling, Ms. Anjani Pandya, Ms. Samantha Mannala, Mr. Shrunjal Trivedi and Ms. Shriya Sarin. We appreciate the talents of our nurses Ms. Kim Brown, and Ms. Stella Weis who drew blood samples for these studies. And the Ghorpade/Borgmann lab members Ms. Satomi Stacy, Mr. Venkata Viswanadh Edara and Ms. Jessica Proulx for their thorough review and editing of this manuscript.
Funding Information:
This work was funded by the National Institute for Minorities Health and Health Disparities (P20MD006882) to Anuja Ghorpade, Ph.D. (PI Project 2) and the J.E.S. Edwards Foundation of Fort Worth, TX. Acknowledgments
Publisher Copyright:
© 2020, Journal of NeuroVirology, Inc.
PY - 2020/6/1
Y1 - 2020/6/1
N2 - Inflammation in people living with HIV (PLWH) correlates with severity of HIV-associated neurocognitive disorders. The objective of this study is to identify blood-based markers of neurocognitive function in a demographic balanced cohort of PLWH. Seven neurocognitive domains were evaluated in 121 seropositive Black/African American, Non-Hispanic White, and White Hispanic men and women using computerized assessments. Associations among standardized neurocognitive function and HIV-related parameters, relevant sociodemographic variables, and inflammation-associated cytokines measured in plasma and cellular supernatants were examined using multivariate and univariate regression models. Outlier and covariate analyses were used to identify and normalize for education level, CD4 T cell count, viral load, CNS and drug abuse comorbidities, which could influence biomarker and neurocognitive function associations. Plasma levels of chemokine (C-C motif) ligand (CCL) 8 significantly associated with memory, complex attention, cognitive flexibility, psychomotor speed, executive function, and processing speed. Plasma tissue inhibitor of metalloproteinases 1 associated with the aforementioned domains except memory and processing speed. In addition, plasma interleukin-23 significantly associated with processing speed and executive function. Analysis of peripheral blood cell culture supernatants revealed no significant markers for neurocognitive function. In this cohort, CD4 T cell count and education level also significantly associated with neurocognitive function. All identified inflammatory biomarkers demonstrated a negative correlation to neurocognitive function. These cytokines have known connections to HIV pathophysiology and are potential biomarkers for neurocognitive function in PLWH with promising clinical applications.
AB - Inflammation in people living with HIV (PLWH) correlates with severity of HIV-associated neurocognitive disorders. The objective of this study is to identify blood-based markers of neurocognitive function in a demographic balanced cohort of PLWH. Seven neurocognitive domains were evaluated in 121 seropositive Black/African American, Non-Hispanic White, and White Hispanic men and women using computerized assessments. Associations among standardized neurocognitive function and HIV-related parameters, relevant sociodemographic variables, and inflammation-associated cytokines measured in plasma and cellular supernatants were examined using multivariate and univariate regression models. Outlier and covariate analyses were used to identify and normalize for education level, CD4 T cell count, viral load, CNS and drug abuse comorbidities, which could influence biomarker and neurocognitive function associations. Plasma levels of chemokine (C-C motif) ligand (CCL) 8 significantly associated with memory, complex attention, cognitive flexibility, psychomotor speed, executive function, and processing speed. Plasma tissue inhibitor of metalloproteinases 1 associated with the aforementioned domains except memory and processing speed. In addition, plasma interleukin-23 significantly associated with processing speed and executive function. Analysis of peripheral blood cell culture supernatants revealed no significant markers for neurocognitive function. In this cohort, CD4 T cell count and education level also significantly associated with neurocognitive function. All identified inflammatory biomarkers demonstrated a negative correlation to neurocognitive function. These cytokines have known connections to HIV pathophysiology and are potential biomarkers for neurocognitive function in PLWH with promising clinical applications.
KW - CNS
KW - Chronic immune activation
KW - HIV-associated neurocognitive disorders (HAND)
KW - Health disparities
UR - http://www.scopus.com/inward/record.url?scp=85082812313&partnerID=8YFLogxK
U2 - 10.1007/s13365-020-00834-3
DO - 10.1007/s13365-020-00834-3
M3 - Article
C2 - 32193795
AN - SCOPUS:85082812313
SN - 1355-0284
VL - 26
SP - 358
EP - 370
JO - Journal of NeuroVirology
JF - Journal of NeuroVirology
IS - 3
ER -