Biosynthesis of himastatin: Assembly line and characterization of three cytochrome P450 enzymes involved in the post-tailoring oxidative steps

Junying Ma; Zhongwen Wang; Hongbo Huang; Minghe Luo; Dianguang Zuo; Bo Wang; Aijun Sun; Yi Qiang Cheng; Changsheng Zhang; Jianhua Ju

doi:10.1002/anie.201102305

Biosynthesis of himastatin: Assembly line and characterization of three cytochrome P450 enzymes involved in the post-tailoring oxidative steps

Junying Ma, Zhongwen Wang, Hongbo Huang, Minghe Luo, Dianguang Zuo, Bo Wang, Aijun Sun, Yi Qiang Cheng, Changsheng Zhang, Jianhua Ju

Research output: Contribution to journal › Article › peer-review

70 Scopus citations

Abstract

It's only natural: The biosynthetic pathway of himastatin from Streptomyces himastatinicus featuring unusual domain organizations is elucidated by genome scanning, generation of in-frame gene deletion mutants, and complementation experiments. Three cytochrome P450s that catalyze novel post-tailoring oxidative reactions (see picture) are characterized.

Original language	English
Pages (from-to)	7797-7802
Number of pages	6
Journal	Angewandte Chemie - International Edition
Volume	50
Issue number	34
DOIs	https://doi.org/10.1002/anie.201102305
State	Published - 16 Aug 2011

Keywords

C-C coupling
biosynthesis
cytochromes
natural products
peptides

Access to Document

10.1002/anie.201102305

Cite this

@article{c8bade65dd224a12a4dbef11d7450626,

title = "Biosynthesis of himastatin: Assembly line and characterization of three cytochrome P450 enzymes involved in the post-tailoring oxidative steps",

abstract = "It's only natural: The biosynthetic pathway of himastatin from Streptomyces himastatinicus featuring unusual domain organizations is elucidated by genome scanning, generation of in-frame gene deletion mutants, and complementation experiments. Three cytochrome P450s that catalyze novel post-tailoring oxidative reactions (see picture) are characterized.",

keywords = "C-C coupling, biosynthesis, cytochromes, natural products, peptides",

author = "Junying Ma and Zhongwen Wang and Hongbo Huang and Minghe Luo and Dianguang Zuo and Bo Wang and Aijun Sun and Cheng, {Yi Qiang} and Changsheng Zhang and Jianhua Ju",

year = "2011",

month = aug,

day = "16",

doi = "10.1002/anie.201102305",

language = "English",

volume = "50",

pages = "7797--7802",

journal = "Angewandte Chemie - International Edition",

issn = "1433-7851",

publisher = "John Wiley and Sons Ltd",

number = "34",

}

TY - JOUR

T1 - Biosynthesis of himastatin

T2 - Assembly line and characterization of three cytochrome P450 enzymes involved in the post-tailoring oxidative steps

AU - Ma, Junying

AU - Wang, Zhongwen

AU - Huang, Hongbo

AU - Luo, Minghe

AU - Zuo, Dianguang

AU - Wang, Bo

AU - Sun, Aijun

AU - Cheng, Yi Qiang

AU - Zhang, Changsheng

AU - Ju, Jianhua

PY - 2011/8/16

Y1 - 2011/8/16

N2 - It's only natural: The biosynthetic pathway of himastatin from Streptomyces himastatinicus featuring unusual domain organizations is elucidated by genome scanning, generation of in-frame gene deletion mutants, and complementation experiments. Three cytochrome P450s that catalyze novel post-tailoring oxidative reactions (see picture) are characterized.

AB - It's only natural: The biosynthetic pathway of himastatin from Streptomyces himastatinicus featuring unusual domain organizations is elucidated by genome scanning, generation of in-frame gene deletion mutants, and complementation experiments. Three cytochrome P450s that catalyze novel post-tailoring oxidative reactions (see picture) are characterized.

KW - C-C coupling

KW - biosynthesis

KW - cytochromes

KW - natural products

KW - peptides

UR - http://www.scopus.com/inward/record.url?scp=80051740572&partnerID=8YFLogxK

U2 - 10.1002/anie.201102305

DO - 10.1002/anie.201102305

M3 - Article

C2 - 21726028

AN - SCOPUS:80051740572

SN - 1433-7851

VL - 50

SP - 7797

EP - 7802

JO - Angewandte Chemie - International Edition

JF - Angewandte Chemie - International Edition

IS - 34

ER -

Biosynthesis of himastatin: Assembly line and characterization of three cytochrome P450 enzymes involved in the post-tailoring oxidative steps

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this