Biological differences in rAAV transduction of airway epithelia in humans and in old world non-human primates

Xiaoming Liu, Meihui Luo, Cyndi Trygg, Ziying Yan, Diana C.M. Lei-Butters, Carolina I. Smith, Anne C. Fischer, Keith Munson, William B. Guggino, Bruce A. Bunnell, John F. Engelhardt

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Abstract

Non-human primates (NHPs) are considered to be among the most relevant animal models for pre-clinical testing of human therapies, on the basis of their close evolutionary relatedness to humans in terms of organ cell biology and physiology. In this study, we sought to investigate whether NHP models accurately reflect the effectiveness of recombinant adeno-associated virus (rAAV)-mediated gene delivery to the airway in humans. In order to do this, we utilized an identical model system of differentiated airway epithelia from Indian Rhesus monkeys and from humans, cultured at an air-liquid interface (ALI). In addition to assessing the biology of rAAV-mediated transduction for three serotypes, we characterized the bioelectric properties as a reference for biological similarities and differences between the cell cultures from the two species. Our results demonstrate that airway epithelia from NHPs and humans have very similar Na+ and Cl- transport properties. In contrast, rAAV transduction of airway epithelia of NHPs demonstrated significant differences to those in humans with regard to the efficiency of apical and/or basal transduction with three rAAV serotypes (AAV1, AAV2, AAV5). These findings suggest that the IndianRhesusmonkey may not be the best model for preclinical testing of rAAV-mediated gene therapy to the airway in humans.

Original languageEnglish
Pages (from-to)2114-2123
Number of pages10
JournalMolecular Therapy
Volume15
Issue number12
DOIs
StatePublished - Dec 2007

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    Liu, X., Luo, M., Trygg, C., Yan, Z., Lei-Butters, D. C. M., Smith, C. I., Fischer, A. C., Munson, K., Guggino, W. B., Bunnell, B. A., & Engelhardt, J. F. (2007). Biological differences in rAAV transduction of airway epithelia in humans and in old world non-human primates. Molecular Therapy, 15(12), 2114-2123. https://doi.org/10.1038/sj.mt.6300277