Biologic properties of mesenchymal stem cells derived from bone marrow and adipose tissue

Reza Izadpanah, Cynthia Trygg, Bindiya Patel, Christopher Kriedt, Jason Dufour, Jeffery M. Gimble, Bruce A. Bunnell

Research output: Contribution to journalArticlepeer-review

267 Scopus citations


The biologic characteristics of mesenchymal stem cells (MSCs) isolated from two distinct tissues, bone marrow and adipose tissue were evaluated in these studies. MSCs derived from human and non-human primate (rhesus monkey) tissue sources were compared. The data indicate that MSCs isolated from rhesus bone marrow (rBMSCs) and human adipose tissue (hASCs) had more similar biologic properties than MSCs of rhesus adipose tissue (rASCs) and human bone marrow MSCs (hBMSCs). Analyses of in vitro growth kinetics revealed shorter doubling time for rBMSCs and hASCs. rBMSCs and hASCs underwent significantly more population doublings than the other MSCs. MSCs from all sources showed a marked decrease in telomerase activity over extended culture; however, they maintained their mean telomere length. All of the MSCs expressed embryonic stem cell markers, Oct-4, Rex-1, and Sox-2 for at least 10 passages. Early populations of MSCs types showed similar multilineage differentiation capability. However, only the rBMSCs and hASCs retain greater differentiation efficiency at higher passages. Overal I in vitro characterization of MSCs from these two species and tissue sources revealed a high level of common biologic properties. However, the results demonstrate clear biologic distinctions, as well.

Original languageEnglish
Pages (from-to)1285-1297
Number of pages13
JournalJournal of Cellular Biochemistry
Issue number5
StatePublished - 1 Dec 2006


  • Adipose tissue
  • Bone marrow
  • Differentiation
  • Mesenchymal stem cells
  • Telomerase
  • Transcription factors


Dive into the research topics of 'Biologic properties of mesenchymal stem cells derived from bone marrow and adipose tissue'. Together they form a unique fingerprint.

Cite this