TY - JOUR
T1 - Bioengineering systems for modulating notch signaling in cardiovascular development, disease, and regeneration
AU - Gomez, Angello Huerta
AU - Joshi, Sanika
AU - Yang, Yong
AU - Tune, Johnathan D.
AU - Zhao, Ming Tao
AU - Yang, Huaxiao
N1 - Funding Information:
Funding: University of North Texas Start-up Grant and Research Seed Grant (RSG-2021-009) to HXY; NIH/NHLBI grant R01 HL155282-01, American Heart Association (AHA) Career Development Award 18CDA34110293, Additional Ventures AVIF and SVRF awards to MTZ.
Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
PY - 2021/10
Y1 - 2021/10
N2 - The Notch intercellular signaling pathways play significant roles in cardiovascular development, disease, and regeneration through modulating cardiovascular cell specification, proliferation, differentiation, and morphogenesis. The dysregulation of Notch signaling leads to malfunction and maldevelopment of the cardiovascular system. Currently, most findings on Notch signaling rely on animal models and a few clinical studies, which significantly bottleneck the understanding of Notch signaling-associated human cardiovascular development and disease. Recent advances in the bioengineering systems and human pluripotent stem cell-derived cardiovascular cells pave the way to decipher the role of Notch signaling in cardiovascular-related cells (endothelial cells, cardiomyocytes, smooth muscle cells, fibroblasts, and immune cells), and intercellular crosstalk in the physiological, pathological, and regenerative context of the complex human cardiovascular system. In this review, we first summarize the significant roles of Notch signaling in individual cardiac cell types. We then cover the bioengineering systems of microfluidics, hydrogel, spheroid, and 3D bioprinting, which are currently being used for modeling and studying Notch signaling in the cardiovascular system. At last, we provide insights into ancillary supports of bioengineering systems, varied types of cardiovascular cells, and advanced characterization approaches in further refining Notch signaling in cardiovascular development, disease, and regeneration.
AB - The Notch intercellular signaling pathways play significant roles in cardiovascular development, disease, and regeneration through modulating cardiovascular cell specification, proliferation, differentiation, and morphogenesis. The dysregulation of Notch signaling leads to malfunction and maldevelopment of the cardiovascular system. Currently, most findings on Notch signaling rely on animal models and a few clinical studies, which significantly bottleneck the understanding of Notch signaling-associated human cardiovascular development and disease. Recent advances in the bioengineering systems and human pluripotent stem cell-derived cardiovascular cells pave the way to decipher the role of Notch signaling in cardiovascular-related cells (endothelial cells, cardiomyocytes, smooth muscle cells, fibroblasts, and immune cells), and intercellular crosstalk in the physiological, pathological, and regenerative context of the complex human cardiovascular system. In this review, we first summarize the significant roles of Notch signaling in individual cardiac cell types. We then cover the bioengineering systems of microfluidics, hydrogel, spheroid, and 3D bioprinting, which are currently being used for modeling and studying Notch signaling in the cardiovascular system. At last, we provide insights into ancillary supports of bioengineering systems, varied types of cardiovascular cells, and advanced characterization approaches in further refining Notch signaling in cardiovascular development, disease, and regeneration.
KW - 3D bioprinting
KW - Bioengineering
KW - Cardiovascular cells
KW - Hydrogel
KW - Microfluidics
KW - Notch signaling
KW - Organoid
KW - Single-cell omics
KW - Spheroid
UR - http://www.scopus.com/inward/record.url?scp=85116675988&partnerID=8YFLogxK
U2 - 10.3390/jcdd8100125
DO - 10.3390/jcdd8100125
M3 - Review article
AN - SCOPUS:85116675988
SN - 2308-3425
VL - 8
JO - Journal of Cardiovascular Development and Disease
JF - Journal of Cardiovascular Development and Disease
IS - 10
M1 - 125
ER -