TY - JOUR
T1 - Binding sites in the EFG1 promoter for transcription factors in a proposed regulatory network
T2 - A functional analysis in the white and opaque phases of Candida albicans
AU - Pujol, Claude
AU - Srikantha, Thyagarajan
AU - Park, Yang Nim
AU - Daniels, Karla J.
AU - Soll, David R.
N1 - Publisher Copyright:
© 2016 Pujol et al.
PY - 2016
Y1 - 2016
N2 - In Candida albicans the transcription factor Efg1, which is differentially expressed in the white phase of the white-opaque transition, is essential for expression of the white phenotype. It is one of six transcription factors included in a proposed interactive transcription network regulating white-opaque switching and maintenance of the alternative phenotypes. Ten sites were identified in the EFG1 promoter that differentially bind one or more of the network transcription factors in the white and/or opaque phase. To explore the functionality of these binding sites in the differential expression of EFG1, we generated targeted deletions of each of the 10 binding sites, combinatorial deletions, and regional deletions using a Renilla reniformis luciferase reporter system. Individually targeted deletion of only four of the 10 sites had minor effects consistent with differential expression of EFG1, and only in the opaque phase. Alternative explanations are considered.
AB - In Candida albicans the transcription factor Efg1, which is differentially expressed in the white phase of the white-opaque transition, is essential for expression of the white phenotype. It is one of six transcription factors included in a proposed interactive transcription network regulating white-opaque switching and maintenance of the alternative phenotypes. Ten sites were identified in the EFG1 promoter that differentially bind one or more of the network transcription factors in the white and/or opaque phase. To explore the functionality of these binding sites in the differential expression of EFG1, we generated targeted deletions of each of the 10 binding sites, combinatorial deletions, and regional deletions using a Renilla reniformis luciferase reporter system. Individually targeted deletion of only four of the 10 sites had minor effects consistent with differential expression of EFG1, and only in the opaque phase. Alternative explanations are considered.
KW - Cis-acting sequences
KW - Differential gene expression
KW - White-opaque transition
UR - http://www.scopus.com/inward/record.url?scp=84973122005&partnerID=8YFLogxK
U2 - 10.1534/g3.116.029785
DO - 10.1534/g3.116.029785
M3 - Article
C2 - 27172219
AN - SCOPUS:84973122005
SN - 2160-1836
VL - 6
SP - 1725
EP - 1737
JO - G3: Genes, Genomes, Genetics
JF - G3: Genes, Genomes, Genetics
IS - 6
ER -