Binding sites in the EFG1 promoter for transcription factors in a proposed regulatory network: A functional analysis in the white and opaque phases of Candida albicans

Claude Pujol, Srikantha Thyagarajan, Yang Nim Park, Karla J. Daniels, David R. Soll

Research output: Contribution to journalArticle

Abstract

In Candida albicans the transcription factor Efg1, which is differentially expressed in the white phase of the white-opaque transition, is essential for expression of the white phenotype. It is one of six transcription factors included in a proposed interactive transcription network regulating white-opaque switching and maintenance of the alternative phenotypes. Ten sites were identified in the EFG1 promoter that differentially bind one or more of the network transcription factors in the white and/or opaque phase. To explore the functionality of these binding sites in the differential expression of EFG1, we generated targeted deletions of each of the 10 binding sites, combinatorial deletions, and regional deletions using a Renilla reniformis luciferase reporter system. Individually targeted deletion of only four of the 10 sites had minor effects consistent with differential expression of EFG1, and only in the opaque phase. Alternative explanations are considered.

Original languageEnglish
Pages (from-to)1725-1737
Number of pages13
JournalG3: Genes, Genomes, Genetics
Volume6
Issue number6
DOIs
StatePublished - 1 Jan 2016

Fingerprint

Candida albicans
Transcription Factors
Binding Sites
Renilla Luciferases
Phenotype
Maintenance

Keywords

  • Cis-acting sequences
  • Differential gene expression
  • White-opaque transition

Cite this

@article{5c83fff810294d338bd9678e3a693bc7,
title = "Binding sites in the EFG1 promoter for transcription factors in a proposed regulatory network: A functional analysis in the white and opaque phases of Candida albicans",
abstract = "In Candida albicans the transcription factor Efg1, which is differentially expressed in the white phase of the white-opaque transition, is essential for expression of the white phenotype. It is one of six transcription factors included in a proposed interactive transcription network regulating white-opaque switching and maintenance of the alternative phenotypes. Ten sites were identified in the EFG1 promoter that differentially bind one or more of the network transcription factors in the white and/or opaque phase. To explore the functionality of these binding sites in the differential expression of EFG1, we generated targeted deletions of each of the 10 binding sites, combinatorial deletions, and regional deletions using a Renilla reniformis luciferase reporter system. Individually targeted deletion of only four of the 10 sites had minor effects consistent with differential expression of EFG1, and only in the opaque phase. Alternative explanations are considered.",
keywords = "Cis-acting sequences, Differential gene expression, White-opaque transition",
author = "Claude Pujol and Srikantha Thyagarajan and Park, {Yang Nim} and Daniels, {Karla J.} and Soll, {David R.}",
year = "2016",
month = "1",
day = "1",
doi = "10.1534/g3.116.029785",
language = "English",
volume = "6",
pages = "1725--1737",
journal = "G3: Genes, Genomes, Genetics",
issn = "2160-1836",
publisher = "Genetics Society of America",
number = "6",

}

Binding sites in the EFG1 promoter for transcription factors in a proposed regulatory network : A functional analysis in the white and opaque phases of Candida albicans. / Pujol, Claude; Thyagarajan, Srikantha; Park, Yang Nim; Daniels, Karla J.; Soll, David R.

In: G3: Genes, Genomes, Genetics, Vol. 6, No. 6, 01.01.2016, p. 1725-1737.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Binding sites in the EFG1 promoter for transcription factors in a proposed regulatory network

T2 - A functional analysis in the white and opaque phases of Candida albicans

AU - Pujol, Claude

AU - Thyagarajan, Srikantha

AU - Park, Yang Nim

AU - Daniels, Karla J.

AU - Soll, David R.

PY - 2016/1/1

Y1 - 2016/1/1

N2 - In Candida albicans the transcription factor Efg1, which is differentially expressed in the white phase of the white-opaque transition, is essential for expression of the white phenotype. It is one of six transcription factors included in a proposed interactive transcription network regulating white-opaque switching and maintenance of the alternative phenotypes. Ten sites were identified in the EFG1 promoter that differentially bind one or more of the network transcription factors in the white and/or opaque phase. To explore the functionality of these binding sites in the differential expression of EFG1, we generated targeted deletions of each of the 10 binding sites, combinatorial deletions, and regional deletions using a Renilla reniformis luciferase reporter system. Individually targeted deletion of only four of the 10 sites had minor effects consistent with differential expression of EFG1, and only in the opaque phase. Alternative explanations are considered.

AB - In Candida albicans the transcription factor Efg1, which is differentially expressed in the white phase of the white-opaque transition, is essential for expression of the white phenotype. It is one of six transcription factors included in a proposed interactive transcription network regulating white-opaque switching and maintenance of the alternative phenotypes. Ten sites were identified in the EFG1 promoter that differentially bind one or more of the network transcription factors in the white and/or opaque phase. To explore the functionality of these binding sites in the differential expression of EFG1, we generated targeted deletions of each of the 10 binding sites, combinatorial deletions, and regional deletions using a Renilla reniformis luciferase reporter system. Individually targeted deletion of only four of the 10 sites had minor effects consistent with differential expression of EFG1, and only in the opaque phase. Alternative explanations are considered.

KW - Cis-acting sequences

KW - Differential gene expression

KW - White-opaque transition

UR - http://www.scopus.com/inward/record.url?scp=84973122005&partnerID=8YFLogxK

U2 - 10.1534/g3.116.029785

DO - 10.1534/g3.116.029785

M3 - Article

C2 - 27172219

AN - SCOPUS:84973122005

VL - 6

SP - 1725

EP - 1737

JO - G3: Genes, Genomes, Genetics

JF - G3: Genes, Genomes, Genetics

SN - 2160-1836

IS - 6

ER -