Abstract
Proteins of the bcl-2 family are important regulators of apoptosis in many tissues of the embryo and adult and may play a role in cell death following stroke. The recently isolated bcl-w gene encodes a pro-survival member of the bcl-2 family, which is widely expressed. However, it is not known whether bcl-w plays a role in determining cell survival after cerebral ischemia. Using Western blot analysis and immunocytochemistry, regional bcl-w protein expression was studied in rat brain 2, 6, 24 and 72 h following 20 min temporary middle cerebral artery occlusion (MCAO). Focal cerebral ischemia increased bcl-w protein expression within the caudate putamen and parietal cortex, as well as causing milder increases within frontal cortex. Immunocytochemically bcl-w was expressed within neurons (frontal and parietal cortex) and glia (caudate putamen) 24 h after MCAO. These data suggest that bcl-w could play a role in determining cell survival after cerebral ischemia. (C) 2000 Elsevier Science Ireland Ltd.
Original language | English |
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Pages (from-to) | 193-195 |
Number of pages | 3 |
Journal | Neuroscience letters |
Volume | 279 |
Issue number | 3 |
DOIs | |
State | Published - 4 Feb 2000 |
Keywords
- Apoptosis
- Bcl-w
- Cerebral ischemia
- Immunocytochemistry
- Neurons
- Programmed cell death
- Western blot analysis