Bcl-w expression is increased in brain regions affected by focal cerebral ischemia in the rat

Manabu Minami; Kun Lin Jin; Wenjin Li; Tetsuya Nagayama; David C. Henshall; Roger P. Simon

doi:10.1016/S0304-3940(99)00987-8

Bcl-w expression is increased in brain regions affected by focal cerebral ischemia in the rat

Manabu Minami, Kun Lin Jin, Wenjin Li, Tetsuya Nagayama, David C. Henshall, Roger P. Simon

Research output: Contribution to journal › Article › peer-review

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Abstract

Proteins of the bcl-2 family are important regulators of apoptosis in many tissues of the embryo and adult and may play a role in cell death following stroke. The recently isolated bcl-w gene encodes a pro-survival member of the bcl-2 family, which is widely expressed. However, it is not known whether bcl-w plays a role in determining cell survival after cerebral ischemia. Using Western blot analysis and immunocytochemistry, regional bcl-w protein expression was studied in rat brain 2, 6, 24 and 72 h following 20 min temporary middle cerebral artery occlusion (MCAO). Focal cerebral ischemia increased bcl-w protein expression within the caudate putamen and parietal cortex, as well as causing milder increases within frontal cortex. Immunocytochemically bcl-w was expressed within neurons (frontal and parietal cortex) and glia (caudate putamen) 24 h after MCAO. These data suggest that bcl-w could play a role in determining cell survival after cerebral ischemia. (C) 2000 Elsevier Science Ireland Ltd.

Original language	English
Pages (from-to)	193-195
Number of pages	3
Journal	Neuroscience letters
Volume	279
Issue number	3
DOIs	https://doi.org/10.1016/S0304-3940(99)00987-8
State	Published - 4 Feb 2000

Keywords

Apoptosis
Bcl-w
Cerebral ischemia
Immunocytochemistry
Neurons
Programmed cell death
Western blot analysis

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10.1016/S0304-3940(99)00987-8

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title = "Bcl-w expression is increased in brain regions affected by focal cerebral ischemia in the rat",

abstract = "Proteins of the bcl-2 family are important regulators of apoptosis in many tissues of the embryo and adult and may play a role in cell death following stroke. The recently isolated bcl-w gene encodes a pro-survival member of the bcl-2 family, which is widely expressed. However, it is not known whether bcl-w plays a role in determining cell survival after cerebral ischemia. Using Western blot analysis and immunocytochemistry, regional bcl-w protein expression was studied in rat brain 2, 6, 24 and 72 h following 20 min temporary middle cerebral artery occlusion (MCAO). Focal cerebral ischemia increased bcl-w protein expression within the caudate putamen and parietal cortex, as well as causing milder increases within frontal cortex. Immunocytochemically bcl-w was expressed within neurons (frontal and parietal cortex) and glia (caudate putamen) 24 h after MCAO. These data suggest that bcl-w could play a role in determining cell survival after cerebral ischemia. (C) 2000 Elsevier Science Ireland Ltd.",

keywords = "Apoptosis, Bcl-w, Cerebral ischemia, Immunocytochemistry, Neurons, Programmed cell death, Western blot analysis",

author = "Manabu Minami and {Lin Jin}, Kun and Wenjin Li and Tetsuya Nagayama and Henshall, {David C.} and Simon, {Roger P.}",

note = "Funding Information: This study was supported by NIH Grant NS24728 (R.P.S.).",

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doi = "10.1016/S0304-3940(99)00987-8",

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T1 - Bcl-w expression is increased in brain regions affected by focal cerebral ischemia in the rat

AU - Minami, Manabu

AU - Lin Jin, Kun

AU - Li, Wenjin

AU - Nagayama, Tetsuya

AU - Henshall, David C.

AU - Simon, Roger P.

N1 - Funding Information: This study was supported by NIH Grant NS24728 (R.P.S.).

PY - 2000/2/4

Y1 - 2000/2/4

N2 - Proteins of the bcl-2 family are important regulators of apoptosis in many tissues of the embryo and adult and may play a role in cell death following stroke. The recently isolated bcl-w gene encodes a pro-survival member of the bcl-2 family, which is widely expressed. However, it is not known whether bcl-w plays a role in determining cell survival after cerebral ischemia. Using Western blot analysis and immunocytochemistry, regional bcl-w protein expression was studied in rat brain 2, 6, 24 and 72 h following 20 min temporary middle cerebral artery occlusion (MCAO). Focal cerebral ischemia increased bcl-w protein expression within the caudate putamen and parietal cortex, as well as causing milder increases within frontal cortex. Immunocytochemically bcl-w was expressed within neurons (frontal and parietal cortex) and glia (caudate putamen) 24 h after MCAO. These data suggest that bcl-w could play a role in determining cell survival after cerebral ischemia. (C) 2000 Elsevier Science Ireland Ltd.

AB - Proteins of the bcl-2 family are important regulators of apoptosis in many tissues of the embryo and adult and may play a role in cell death following stroke. The recently isolated bcl-w gene encodes a pro-survival member of the bcl-2 family, which is widely expressed. However, it is not known whether bcl-w plays a role in determining cell survival after cerebral ischemia. Using Western blot analysis and immunocytochemistry, regional bcl-w protein expression was studied in rat brain 2, 6, 24 and 72 h following 20 min temporary middle cerebral artery occlusion (MCAO). Focal cerebral ischemia increased bcl-w protein expression within the caudate putamen and parietal cortex, as well as causing milder increases within frontal cortex. Immunocytochemically bcl-w was expressed within neurons (frontal and parietal cortex) and glia (caudate putamen) 24 h after MCAO. These data suggest that bcl-w could play a role in determining cell survival after cerebral ischemia. (C) 2000 Elsevier Science Ireland Ltd.

KW - Apoptosis

KW - Bcl-w

KW - Cerebral ischemia

KW - Immunocytochemistry

KW - Neurons

KW - Programmed cell death

KW - Western blot analysis

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DO - 10.1016/S0304-3940(99)00987-8

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AN - SCOPUS:0343851673

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SP - 193

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JO - Neuroscience Letters

JF - Neuroscience Letters

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ER -

Bcl-w expression is increased in brain regions affected by focal cerebral ischemia in the rat

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