Bcl-w expression is increased in brain regions affected by focal cerebral ischemia in the rat

Manabu Minami, Kun Lin Jin, Wenjin Li, Tetsuya Nagayama, David C. Henshall, Roger P. Simon

Research output: Contribution to journalArticle

33 Scopus citations

Abstract

Proteins of the bcl-2 family are important regulators of apoptosis in many tissues of the embryo and adult and may play a role in cell death following stroke. The recently isolated bcl-w gene encodes a pro-survival member of the bcl-2 family, which is widely expressed. However, it is not known whether bcl-w plays a role in determining cell survival after cerebral ischemia. Using Western blot analysis and immunocytochemistry, regional bcl-w protein expression was studied in rat brain 2, 6, 24 and 72 h following 20 min temporary middle cerebral artery occlusion (MCAO). Focal cerebral ischemia increased bcl-w protein expression within the caudate putamen and parietal cortex, as well as causing milder increases within frontal cortex. Immunocytochemically bcl-w was expressed within neurons (frontal and parietal cortex) and glia (caudate putamen) 24 h after MCAO. These data suggest that bcl-w could play a role in determining cell survival after cerebral ischemia. (C) 2000 Elsevier Science Ireland Ltd.

Original languageEnglish
Pages (from-to)193-195
Number of pages3
JournalNeuroscience Letters
Volume279
Issue number3
DOIs
StatePublished - 4 Feb 2000

Keywords

  • Apoptosis
  • Bcl-w
  • Cerebral ischemia
  • Immunocytochemistry
  • Neurons
  • Programmed cell death
  • Western blot analysis

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