Auto-fluorescence lifetime and light reflectance spectroscopy for breast cancer diagnosis: Potential tools for intraoperative margin detection

Vikrant Sharma, Shivaranjani Shivalingaiah, Yan Peng, David Euhus, Zygmunt Gryczynski, Hanli Liu

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

This study investigates the use of two spectroscopic techniques, auto-fluorescence lifetime measurement (AFLM) and light reflectance spectroscopy (LRS), for detecting invasive ductal carcinoma (IDC) in human ex vivo breast specimens. AFLM used excitation at 447 nm with multiple emission wavelengths (532, 562, 632, and 644 nm), at which auto-fluorescence lifetimes and their weight factors were analyzed using a double exponent model. LRS measured reflectance spectra in the range of 500-840 nm and analyzed the spectral slopes empirically at several distinct spectral regions. Our preliminary results based on 93 measured locations (i.e., 34 IDC, 31 benign fibrous, 28 adipose) from 6 specimens show significant differences in 5 AFLM-derived parameters and 9 LRS-based spectral slopes between benign and malignant breast samples. Multinomial logistic regression with a 10-fold cross validation approach was implemented with selected features to classify IDC from benign fibrous and adipose tissues for the two techniques independently as well as for the combined dual-modality approach. The accuracy for classifying IDC was found to be 96.4 ± 0.8%, 92.3 ± 0.8% and 96 ± 1.3% for LRS, AFLM, and dual-modality, respectively.

Original languageEnglish
Pages (from-to)1825-1840
Number of pages16
JournalBiomedical Optics Express
Volume3
Issue number8
DOIs
StatePublished - 1 Aug 2012

Fingerprint

Ductal Carcinoma
breast
margins
Spectrum Analysis
Fluorescence
cancer
Breast Neoplasms
reflectance
Light
life (durability)
fluorescence
spectroscopy
Breast
adipose tissues
Fluorescence Spectrometry
slopes
weight (mass)
logistics
Adipose Tissue
classifying

Cite this

Sharma, Vikrant ; Shivalingaiah, Shivaranjani ; Peng, Yan ; Euhus, David ; Gryczynski, Zygmunt ; Liu, Hanli. / Auto-fluorescence lifetime and light reflectance spectroscopy for breast cancer diagnosis : Potential tools for intraoperative margin detection. In: Biomedical Optics Express. 2012 ; Vol. 3, No. 8. pp. 1825-1840.
@article{f0df0d62f2bf43139b942915e3cd25f5,
title = "Auto-fluorescence lifetime and light reflectance spectroscopy for breast cancer diagnosis: Potential tools for intraoperative margin detection",
abstract = "This study investigates the use of two spectroscopic techniques, auto-fluorescence lifetime measurement (AFLM) and light reflectance spectroscopy (LRS), for detecting invasive ductal carcinoma (IDC) in human ex vivo breast specimens. AFLM used excitation at 447 nm with multiple emission wavelengths (532, 562, 632, and 644 nm), at which auto-fluorescence lifetimes and their weight factors were analyzed using a double exponent model. LRS measured reflectance spectra in the range of 500-840 nm and analyzed the spectral slopes empirically at several distinct spectral regions. Our preliminary results based on 93 measured locations (i.e., 34 IDC, 31 benign fibrous, 28 adipose) from 6 specimens show significant differences in 5 AFLM-derived parameters and 9 LRS-based spectral slopes between benign and malignant breast samples. Multinomial logistic regression with a 10-fold cross validation approach was implemented with selected features to classify IDC from benign fibrous and adipose tissues for the two techniques independently as well as for the combined dual-modality approach. The accuracy for classifying IDC was found to be 96.4 ± 0.8{\%}, 92.3 ± 0.8{\%} and 96 ± 1.3{\%} for LRS, AFLM, and dual-modality, respectively.",
author = "Vikrant Sharma and Shivaranjani Shivalingaiah and Yan Peng and David Euhus and Zygmunt Gryczynski and Hanli Liu",
year = "2012",
month = "8",
day = "1",
doi = "10.1364/BOE.3.001825",
language = "English",
volume = "3",
pages = "1825--1840",
journal = "Biomedical Optics Express",
issn = "2156-7085",
publisher = "The Optical Society",
number = "8",

}

Auto-fluorescence lifetime and light reflectance spectroscopy for breast cancer diagnosis : Potential tools for intraoperative margin detection. / Sharma, Vikrant; Shivalingaiah, Shivaranjani; Peng, Yan; Euhus, David; Gryczynski, Zygmunt; Liu, Hanli.

In: Biomedical Optics Express, Vol. 3, No. 8, 01.08.2012, p. 1825-1840.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Auto-fluorescence lifetime and light reflectance spectroscopy for breast cancer diagnosis

T2 - Potential tools for intraoperative margin detection

AU - Sharma, Vikrant

AU - Shivalingaiah, Shivaranjani

AU - Peng, Yan

AU - Euhus, David

AU - Gryczynski, Zygmunt

AU - Liu, Hanli

PY - 2012/8/1

Y1 - 2012/8/1

N2 - This study investigates the use of two spectroscopic techniques, auto-fluorescence lifetime measurement (AFLM) and light reflectance spectroscopy (LRS), for detecting invasive ductal carcinoma (IDC) in human ex vivo breast specimens. AFLM used excitation at 447 nm with multiple emission wavelengths (532, 562, 632, and 644 nm), at which auto-fluorescence lifetimes and their weight factors were analyzed using a double exponent model. LRS measured reflectance spectra in the range of 500-840 nm and analyzed the spectral slopes empirically at several distinct spectral regions. Our preliminary results based on 93 measured locations (i.e., 34 IDC, 31 benign fibrous, 28 adipose) from 6 specimens show significant differences in 5 AFLM-derived parameters and 9 LRS-based spectral slopes between benign and malignant breast samples. Multinomial logistic regression with a 10-fold cross validation approach was implemented with selected features to classify IDC from benign fibrous and adipose tissues for the two techniques independently as well as for the combined dual-modality approach. The accuracy for classifying IDC was found to be 96.4 ± 0.8%, 92.3 ± 0.8% and 96 ± 1.3% for LRS, AFLM, and dual-modality, respectively.

AB - This study investigates the use of two spectroscopic techniques, auto-fluorescence lifetime measurement (AFLM) and light reflectance spectroscopy (LRS), for detecting invasive ductal carcinoma (IDC) in human ex vivo breast specimens. AFLM used excitation at 447 nm with multiple emission wavelengths (532, 562, 632, and 644 nm), at which auto-fluorescence lifetimes and their weight factors were analyzed using a double exponent model. LRS measured reflectance spectra in the range of 500-840 nm and analyzed the spectral slopes empirically at several distinct spectral regions. Our preliminary results based on 93 measured locations (i.e., 34 IDC, 31 benign fibrous, 28 adipose) from 6 specimens show significant differences in 5 AFLM-derived parameters and 9 LRS-based spectral slopes between benign and malignant breast samples. Multinomial logistic regression with a 10-fold cross validation approach was implemented with selected features to classify IDC from benign fibrous and adipose tissues for the two techniques independently as well as for the combined dual-modality approach. The accuracy for classifying IDC was found to be 96.4 ± 0.8%, 92.3 ± 0.8% and 96 ± 1.3% for LRS, AFLM, and dual-modality, respectively.

UR - http://www.scopus.com/inward/record.url?scp=84865618409&partnerID=8YFLogxK

U2 - 10.1364/BOE.3.001825

DO - 10.1364/BOE.3.001825

M3 - Article

C2 - 22876347

AN - SCOPUS:84865618409

VL - 3

SP - 1825

EP - 1840

JO - Biomedical Optics Express

JF - Biomedical Optics Express

SN - 2156-7085

IS - 8

ER -