Attenuation of ischemia-induced rat brain injury by 2-(-2-benzofuranyl)-2- imidazoline, a high selectivity ligand for imidazoline I2 receptors

Zhao Han, Mei Juan Xiao, Bei Shao, Rong Yuan Zheng, Guo Yuan Yang, Kunlin Jin

Research output: Contribution to journalArticlepeer-review

25 Scopus citations

Abstract

Objective: The aim of this study was to determine whether 2-(2-benzofuranyl)-2-imidazoline, an imidazoline I2 receptor ligand, could protect against cell death from brain injury and improve the functional outcome after focal cerebral ischemia in rats. Methods: Transient focal ischemia was induced by suture occlusion of the middle cerebral artery. Rats were intraperitoneally treated with a vehicle, 2-(2-benzofuranyl)-2-imidazoline or idazoxan immediately after focal ischemia. Infarct volume was assessed by 2,3,5-triphenyltrazolium chloride staining and neurobehavioral deficits were monitored. The volume of cell death in the penumbra after ischemia was determined by immunostaining using anti-cleaved caspase-3 antibody and terminal deoxynucleotidyl transferase biotin-dUTP nick end labeling (TUNEL). Results: Both 2-(2-benzofuranyl)-2-imidazoline and idazoxan significantly improved the neurological score compared with the vehicle at 24 hours after focal ischemia. Treatment with 2-(2-benzofuranyl)-2-imidazoline or idazoxan also significantly reduced infarct volume and the number of both caspase-3- and TUNEL-positive cells in the penumbra compared with vehicle-treated rats (p,0.01 and p,0.05, respectively). Conclusion: The results suggest the neuroprotective role of 2-(2-benzofuranyl)-2-imidazoline and idazoxan in focal cerebral ischemia, and may therefore represent useful targets for developing new treatments for stroke.

Original languageEnglish
Pages (from-to)390-395
Number of pages6
JournalNeurological Research
Volume31
Issue number4
DOIs
StatePublished - 1 May 2009

Keywords

  • Brain
  • Cell death
  • Idazoxan
  • Middle cerebral artery occlusion
  • Rat
  • TUNEL

Fingerprint Dive into the research topics of 'Attenuation of ischemia-induced rat brain injury by 2-(-2-benzofuranyl)-2- imidazoline, a high selectivity ligand for imidazoline I<sub>2</sub> receptors'. Together they form a unique fingerprint.

Cite this