Attenuated mesangial cell proliferation related to store-operated Ca 2+ entry in aged rat

The role of STIM 1 and Orai 1

Bing Shen, Jinhang Zhu, Jin Zhang, Feifei Jiang, Zhaoyi Wang, Yang Zhang, Jie Li, Dake Huang, Daoping Ke, Rong Ma, Juan Du

Research output: Contribution to journalArticleResearchpeer-review

11 Citations (Scopus)

Abstract

Store-operated Ca 2+ entry (SOCE) is a common and ubiquitous mechanism regulating Ca 2+ influx into cells and participates in numerous biological processes including cell proliferation. Glomerular mesangial cells (GMCs) play a role in the regulation of the glomerular filtration rate. From a clinical point of view, many physiological functions alter with age. In the present study, we used angiotensin II, glucagonand the sarco/endoplasmic reticulum membrane Ca 2+ pump inhibitor thapsigargin to deplete the internal Ca 2+ stores for the activation of SOCE. We found that SOCE was significantly attenuated in GMCs from aged (22-month-old) rats. The expression of SOCErelated components, stromal interaction molecule 1 (STIM 1) and Orai 1, in freshly isolated glomeruli notably decreased, and STIM 1 and Orai 1 puncta formation significantly reduced in primary-cultured GMCs in aged rats. Moreover, specific knockdown of STIM 1 and Orai 1 by small interfering RNA markedly suppressed SOCE and cell proliferation of GMCs isolated from young (3-month-old) rats. We conclude that the attenuation of GMCs proliferation can be attributed to the decreased SOCE partially caused by reduced expression of STIM 1 and Orai 1.

Original languageEnglish
Pages (from-to)2193-2202
Number of pages10
JournalAge
Volume35
Issue number6
DOIs
StatePublished - 1 Dec 2013

Fingerprint

Mesangial Cells
Cell Proliferation
Biological Phenomena
Thapsigargin
Glomerular Filtration Rate
Angiotensin II
Endoplasmic Reticulum
Small Interfering RNA
Stromal Interaction Molecule 1
Membranes

Keywords

  • Aging
  • Glomerular mesangial cell
  • Orai 1
  • Proliferation
  • STIM 1

Cite this

Shen, Bing ; Zhu, Jinhang ; Zhang, Jin ; Jiang, Feifei ; Wang, Zhaoyi ; Zhang, Yang ; Li, Jie ; Huang, Dake ; Ke, Daoping ; Ma, Rong ; Du, Juan. / Attenuated mesangial cell proliferation related to store-operated Ca 2+ entry in aged rat : The role of STIM 1 and Orai 1. In: Age. 2013 ; Vol. 35, No. 6. pp. 2193-2202.
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abstract = "Store-operated Ca 2+ entry (SOCE) is a common and ubiquitous mechanism regulating Ca 2+ influx into cells and participates in numerous biological processes including cell proliferation. Glomerular mesangial cells (GMCs) play a role in the regulation of the glomerular filtration rate. From a clinical point of view, many physiological functions alter with age. In the present study, we used angiotensin II, glucagonand the sarco/endoplasmic reticulum membrane Ca 2+ pump inhibitor thapsigargin to deplete the internal Ca 2+ stores for the activation of SOCE. We found that SOCE was significantly attenuated in GMCs from aged (22-month-old) rats. The expression of SOCErelated components, stromal interaction molecule 1 (STIM 1) and Orai 1, in freshly isolated glomeruli notably decreased, and STIM 1 and Orai 1 puncta formation significantly reduced in primary-cultured GMCs in aged rats. Moreover, specific knockdown of STIM 1 and Orai 1 by small interfering RNA markedly suppressed SOCE and cell proliferation of GMCs isolated from young (3-month-old) rats. We conclude that the attenuation of GMCs proliferation can be attributed to the decreased SOCE partially caused by reduced expression of STIM 1 and Orai 1.",
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Shen, B, Zhu, J, Zhang, J, Jiang, F, Wang, Z, Zhang, Y, Li, J, Huang, D, Ke, D, Ma, R & Du, J 2013, 'Attenuated mesangial cell proliferation related to store-operated Ca 2+ entry in aged rat: The role of STIM 1 and Orai 1', Age, vol. 35, no. 6, pp. 2193-2202. https://doi.org/10.1007/s11357-013-9511-5

Attenuated mesangial cell proliferation related to store-operated Ca 2+ entry in aged rat : The role of STIM 1 and Orai 1. / Shen, Bing; Zhu, Jinhang; Zhang, Jin; Jiang, Feifei; Wang, Zhaoyi; Zhang, Yang; Li, Jie; Huang, Dake; Ke, Daoping; Ma, Rong; Du, Juan.

In: Age, Vol. 35, No. 6, 01.12.2013, p. 2193-2202.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Attenuated mesangial cell proliferation related to store-operated Ca 2+ entry in aged rat

T2 - The role of STIM 1 and Orai 1

AU - Shen, Bing

AU - Zhu, Jinhang

AU - Zhang, Jin

AU - Jiang, Feifei

AU - Wang, Zhaoyi

AU - Zhang, Yang

AU - Li, Jie

AU - Huang, Dake

AU - Ke, Daoping

AU - Ma, Rong

AU - Du, Juan

PY - 2013/12/1

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N2 - Store-operated Ca 2+ entry (SOCE) is a common and ubiquitous mechanism regulating Ca 2+ influx into cells and participates in numerous biological processes including cell proliferation. Glomerular mesangial cells (GMCs) play a role in the regulation of the glomerular filtration rate. From a clinical point of view, many physiological functions alter with age. In the present study, we used angiotensin II, glucagonand the sarco/endoplasmic reticulum membrane Ca 2+ pump inhibitor thapsigargin to deplete the internal Ca 2+ stores for the activation of SOCE. We found that SOCE was significantly attenuated in GMCs from aged (22-month-old) rats. The expression of SOCErelated components, stromal interaction molecule 1 (STIM 1) and Orai 1, in freshly isolated glomeruli notably decreased, and STIM 1 and Orai 1 puncta formation significantly reduced in primary-cultured GMCs in aged rats. Moreover, specific knockdown of STIM 1 and Orai 1 by small interfering RNA markedly suppressed SOCE and cell proliferation of GMCs isolated from young (3-month-old) rats. We conclude that the attenuation of GMCs proliferation can be attributed to the decreased SOCE partially caused by reduced expression of STIM 1 and Orai 1.

AB - Store-operated Ca 2+ entry (SOCE) is a common and ubiquitous mechanism regulating Ca 2+ influx into cells and participates in numerous biological processes including cell proliferation. Glomerular mesangial cells (GMCs) play a role in the regulation of the glomerular filtration rate. From a clinical point of view, many physiological functions alter with age. In the present study, we used angiotensin II, glucagonand the sarco/endoplasmic reticulum membrane Ca 2+ pump inhibitor thapsigargin to deplete the internal Ca 2+ stores for the activation of SOCE. We found that SOCE was significantly attenuated in GMCs from aged (22-month-old) rats. The expression of SOCErelated components, stromal interaction molecule 1 (STIM 1) and Orai 1, in freshly isolated glomeruli notably decreased, and STIM 1 and Orai 1 puncta formation significantly reduced in primary-cultured GMCs in aged rats. Moreover, specific knockdown of STIM 1 and Orai 1 by small interfering RNA markedly suppressed SOCE and cell proliferation of GMCs isolated from young (3-month-old) rats. We conclude that the attenuation of GMCs proliferation can be attributed to the decreased SOCE partially caused by reduced expression of STIM 1 and Orai 1.

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KW - Proliferation

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U2 - 10.1007/s11357-013-9511-5

DO - 10.1007/s11357-013-9511-5

M3 - Article

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JO - Age

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SN - 0161-9152

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