Attenuated mesangial cell proliferation related to store-operated Ca 2+ entry in aged rat: The role of STIM 1 and Orai 1

Bing Shen, Jinhang Zhu, Jin Zhang, Feifei Jiang, Zhaoyi Wang, Yang Zhang, Jie Li, Dake Huang, Daoping Ke, Rong Ma, Juan Du

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

Store-operated Ca2+ entry (SOCE) is a common and ubiquitous mechanism regulating Ca2+ influx into cells and participates in numerous biological processes including cell proliferation. Glomerular mesangial cells (GMCs) play a role in the regulation of the glomerular filtration rate. From a clinical point of view, many physiological functions alter with age. In the present study, we used angiotensin II, glucagonand the sarco/endoplasmic reticulum membrane Ca2+ pump inhibitor thapsigargin to deplete the internal Ca2+ stores for the activation of SOCE. We found that SOCE was significantly attenuated in GMCs from aged (22-month-old) rats. The expression of SOCErelated components, stromal interaction molecule 1 (STIM 1) and Orai 1, in freshly isolated glomeruli notably decreased, and STIM 1 and Orai 1 puncta formation significantly reduced in primary-cultured GMCs in aged rats. Moreover, specific knockdown of STIM 1 and Orai 1 by small interfering RNA markedly suppressed SOCE and cell proliferation of GMCs isolated from young (3-month-old) rats. We conclude that the attenuation of GMCs proliferation can be attributed to the decreased SOCE partially caused by reduced expression of STIM 1 and Orai 1.

Original languageEnglish
Pages (from-to)2193-2202
Number of pages10
JournalAge
Volume35
Issue number6
DOIs
StatePublished - Dec 2013

Keywords

  • Aging
  • Glomerular mesangial cell
  • Orai 1
  • Proliferation
  • STIM 1

Fingerprint Dive into the research topics of 'Attenuated mesangial cell proliferation related to store-operated Ca <sup>2+</sup> entry in aged rat: The role of STIM 1 and Orai 1'. Together they form a unique fingerprint.

Cite this