Atrial fibrillation and electrophysiology in transgenic mice with cardiac-restricted overexpression of FKBP12

Zhenwei Pan, Tomohiko Ai, Po Cheng Chang, Ying Liu, Jijia Liu, Mitsunori Maruyama, Mohamed Homsi, Michael C. Fishbein, Michael Rubart, Shien Fong Lin, Deyong Xiao, Hanying Chen, Peng Sheng Chen, Weinian Shou, Bai Yan Li

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Cardiomyocyte-restricted overexpression of FK506-binding protein 12 transgenic (αMyHC-FKBP12) mice develop spontaneous atrial fibrillation (AF). The aim of the present study is to explore the mechanisms underlying the occurrence of AF in αMyHC-FKBP12 mice. Spontaneous AF was documented by telemetry in vivo and Langendorff-perfused hearts of αMyHC-FKBP12 and littermate control mice in vitro. Atrial conduction velocity was evaluated by optical mapping. The patch-clamp technique was applied to determine the potentially altered electrophysiology in atrial myocytes. Channel protein expression levels were evaluated by Western blot analyses. Spontaneous AF was recorded in four of seven αMyHC-FKBP12 mice but in none of eight nontransgenic (NTG) controls. Atrial conduction velocity was significantly reduced in αMyHC-FKBP12 hearts compared with NTG hearts. Interestingly, the mean action potential duration at 50% but not 90% was significantly prolonged in αMyHC-FKBP12 atrial myocytes compared with their NTG counterparts. Consistent with decreased conduction velocity, average peak Na + current (INa) density was dramatically reduced and the INa inactivation curve was shifted by approximately +7 mV in αMyHC-FKBP12 atrial myocytes, whereas the activation and recovery curves were unaltered. The Nav1.5 expression level was significantly reduced in αMyHC-FKBP12 atria. Furthermore, we found increases in atrial Cav1.2 protein levels and peak L-type Ca 2+ current density and increased levels of fibrosis in αMyHC-FKBP12 atria. In summary, cardiomyocyte-restricted overexpression of FKBP12 reduces the atrial Nav1.5 expression level and mean peak INa, which is associated with increased peak L-type Ca 2+ current and interstitial fibrosis in atria. The combined electrophysiological and structural changes facilitated the development of local conduction block and altered action potential duration and spontaneous AF. NEW & NOTEWORTHY This study addresses a long-standing riddle regarding the role of FK506-binding protein 12 in cardiac physiology. The work provides further evidence that FK506-binding protein 12 is a critical component for regulating voltage-gated sodium current and in so doing has an important role in arrhythmogenic physiology, such as atrial fibrillation.

Original languageEnglish
Pages (from-to)H371-H379
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume316
Issue number2
DOIs
StatePublished - 1 Feb 2019

Fingerprint

Tacrolimus Binding Protein 1A
Electrophysiology
Atrial Fibrillation
Transgenic Mice
Tacrolimus Binding Proteins
Muscle Cells
Cardiac Myocytes
Action Potentials
Fibrosis
Telemetry
Patch-Clamp Techniques
Proteins

Keywords

  • Action potential duration
  • Cardiac electrophysiology
  • Fibrosis
  • Ion channels
  • Patch clamp
  • Voltage-gated sodium current

Cite this

Pan, Zhenwei ; Ai, Tomohiko ; Chang, Po Cheng ; Liu, Ying ; Liu, Jijia ; Maruyama, Mitsunori ; Homsi, Mohamed ; Fishbein, Michael C. ; Rubart, Michael ; Lin, Shien Fong ; Xiao, Deyong ; Chen, Hanying ; Chen, Peng Sheng ; Shou, Weinian ; Li, Bai Yan. / Atrial fibrillation and electrophysiology in transgenic mice with cardiac-restricted overexpression of FKBP12. In: American Journal of Physiology - Heart and Circulatory Physiology. 2019 ; Vol. 316, No. 2. pp. H371-H379.
@article{da0f0138a50d4077a7ca5edf86449425,
title = "Atrial fibrillation and electrophysiology in transgenic mice with cardiac-restricted overexpression of FKBP12",
abstract = "Cardiomyocyte-restricted overexpression of FK506-binding protein 12 transgenic (αMyHC-FKBP12) mice develop spontaneous atrial fibrillation (AF). The aim of the present study is to explore the mechanisms underlying the occurrence of AF in αMyHC-FKBP12 mice. Spontaneous AF was documented by telemetry in vivo and Langendorff-perfused hearts of αMyHC-FKBP12 and littermate control mice in vitro. Atrial conduction velocity was evaluated by optical mapping. The patch-clamp technique was applied to determine the potentially altered electrophysiology in atrial myocytes. Channel protein expression levels were evaluated by Western blot analyses. Spontaneous AF was recorded in four of seven αMyHC-FKBP12 mice but in none of eight nontransgenic (NTG) controls. Atrial conduction velocity was significantly reduced in αMyHC-FKBP12 hearts compared with NTG hearts. Interestingly, the mean action potential duration at 50{\%} but not 90{\%} was significantly prolonged in αMyHC-FKBP12 atrial myocytes compared with their NTG counterparts. Consistent with decreased conduction velocity, average peak Na + current (INa) density was dramatically reduced and the INa inactivation curve was shifted by approximately +7 mV in αMyHC-FKBP12 atrial myocytes, whereas the activation and recovery curves were unaltered. The Nav1.5 expression level was significantly reduced in αMyHC-FKBP12 atria. Furthermore, we found increases in atrial Cav1.2 protein levels and peak L-type Ca 2+ current density and increased levels of fibrosis in αMyHC-FKBP12 atria. In summary, cardiomyocyte-restricted overexpression of FKBP12 reduces the atrial Nav1.5 expression level and mean peak INa, which is associated with increased peak L-type Ca 2+ current and interstitial fibrosis in atria. The combined electrophysiological and structural changes facilitated the development of local conduction block and altered action potential duration and spontaneous AF. NEW & NOTEWORTHY This study addresses a long-standing riddle regarding the role of FK506-binding protein 12 in cardiac physiology. The work provides further evidence that FK506-binding protein 12 is a critical component for regulating voltage-gated sodium current and in so doing has an important role in arrhythmogenic physiology, such as atrial fibrillation.",
keywords = "Action potential duration, Cardiac electrophysiology, Fibrosis, Ion channels, Patch clamp, Voltage-gated sodium current",
author = "Zhenwei Pan and Tomohiko Ai and Chang, {Po Cheng} and Ying Liu and Jijia Liu and Mitsunori Maruyama and Mohamed Homsi and Fishbein, {Michael C.} and Michael Rubart and Lin, {Shien Fong} and Deyong Xiao and Hanying Chen and Chen, {Peng Sheng} and Weinian Shou and Li, {Bai Yan}",
year = "2019",
month = "2",
day = "1",
doi = "10.1152/ajpheart.00486.2018",
language = "English",
volume = "316",
pages = "H371--H379",
journal = "American Journal of Physiology - Heart and Circulatory Physiology",
issn = "0363-6135",
publisher = "American Physiological Society",
number = "2",

}

Pan, Z, Ai, T, Chang, PC, Liu, Y, Liu, J, Maruyama, M, Homsi, M, Fishbein, MC, Rubart, M, Lin, SF, Xiao, D, Chen, H, Chen, PS, Shou, W & Li, BY 2019, 'Atrial fibrillation and electrophysiology in transgenic mice with cardiac-restricted overexpression of FKBP12' American Journal of Physiology - Heart and Circulatory Physiology, vol. 316, no. 2, pp. H371-H379. https://doi.org/10.1152/ajpheart.00486.2018

Atrial fibrillation and electrophysiology in transgenic mice with cardiac-restricted overexpression of FKBP12. / Pan, Zhenwei; Ai, Tomohiko; Chang, Po Cheng; Liu, Ying; Liu, Jijia; Maruyama, Mitsunori; Homsi, Mohamed; Fishbein, Michael C.; Rubart, Michael; Lin, Shien Fong; Xiao, Deyong; Chen, Hanying; Chen, Peng Sheng; Shou, Weinian; Li, Bai Yan.

In: American Journal of Physiology - Heart and Circulatory Physiology, Vol. 316, No. 2, 01.02.2019, p. H371-H379.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Atrial fibrillation and electrophysiology in transgenic mice with cardiac-restricted overexpression of FKBP12

AU - Pan, Zhenwei

AU - Ai, Tomohiko

AU - Chang, Po Cheng

AU - Liu, Ying

AU - Liu, Jijia

AU - Maruyama, Mitsunori

AU - Homsi, Mohamed

AU - Fishbein, Michael C.

AU - Rubart, Michael

AU - Lin, Shien Fong

AU - Xiao, Deyong

AU - Chen, Hanying

AU - Chen, Peng Sheng

AU - Shou, Weinian

AU - Li, Bai Yan

PY - 2019/2/1

Y1 - 2019/2/1

N2 - Cardiomyocyte-restricted overexpression of FK506-binding protein 12 transgenic (αMyHC-FKBP12) mice develop spontaneous atrial fibrillation (AF). The aim of the present study is to explore the mechanisms underlying the occurrence of AF in αMyHC-FKBP12 mice. Spontaneous AF was documented by telemetry in vivo and Langendorff-perfused hearts of αMyHC-FKBP12 and littermate control mice in vitro. Atrial conduction velocity was evaluated by optical mapping. The patch-clamp technique was applied to determine the potentially altered electrophysiology in atrial myocytes. Channel protein expression levels were evaluated by Western blot analyses. Spontaneous AF was recorded in four of seven αMyHC-FKBP12 mice but in none of eight nontransgenic (NTG) controls. Atrial conduction velocity was significantly reduced in αMyHC-FKBP12 hearts compared with NTG hearts. Interestingly, the mean action potential duration at 50% but not 90% was significantly prolonged in αMyHC-FKBP12 atrial myocytes compared with their NTG counterparts. Consistent with decreased conduction velocity, average peak Na + current (INa) density was dramatically reduced and the INa inactivation curve was shifted by approximately +7 mV in αMyHC-FKBP12 atrial myocytes, whereas the activation and recovery curves were unaltered. The Nav1.5 expression level was significantly reduced in αMyHC-FKBP12 atria. Furthermore, we found increases in atrial Cav1.2 protein levels and peak L-type Ca 2+ current density and increased levels of fibrosis in αMyHC-FKBP12 atria. In summary, cardiomyocyte-restricted overexpression of FKBP12 reduces the atrial Nav1.5 expression level and mean peak INa, which is associated with increased peak L-type Ca 2+ current and interstitial fibrosis in atria. The combined electrophysiological and structural changes facilitated the development of local conduction block and altered action potential duration and spontaneous AF. NEW & NOTEWORTHY This study addresses a long-standing riddle regarding the role of FK506-binding protein 12 in cardiac physiology. The work provides further evidence that FK506-binding protein 12 is a critical component for regulating voltage-gated sodium current and in so doing has an important role in arrhythmogenic physiology, such as atrial fibrillation.

AB - Cardiomyocyte-restricted overexpression of FK506-binding protein 12 transgenic (αMyHC-FKBP12) mice develop spontaneous atrial fibrillation (AF). The aim of the present study is to explore the mechanisms underlying the occurrence of AF in αMyHC-FKBP12 mice. Spontaneous AF was documented by telemetry in vivo and Langendorff-perfused hearts of αMyHC-FKBP12 and littermate control mice in vitro. Atrial conduction velocity was evaluated by optical mapping. The patch-clamp technique was applied to determine the potentially altered electrophysiology in atrial myocytes. Channel protein expression levels were evaluated by Western blot analyses. Spontaneous AF was recorded in four of seven αMyHC-FKBP12 mice but in none of eight nontransgenic (NTG) controls. Atrial conduction velocity was significantly reduced in αMyHC-FKBP12 hearts compared with NTG hearts. Interestingly, the mean action potential duration at 50% but not 90% was significantly prolonged in αMyHC-FKBP12 atrial myocytes compared with their NTG counterparts. Consistent with decreased conduction velocity, average peak Na + current (INa) density was dramatically reduced and the INa inactivation curve was shifted by approximately +7 mV in αMyHC-FKBP12 atrial myocytes, whereas the activation and recovery curves were unaltered. The Nav1.5 expression level was significantly reduced in αMyHC-FKBP12 atria. Furthermore, we found increases in atrial Cav1.2 protein levels and peak L-type Ca 2+ current density and increased levels of fibrosis in αMyHC-FKBP12 atria. In summary, cardiomyocyte-restricted overexpression of FKBP12 reduces the atrial Nav1.5 expression level and mean peak INa, which is associated with increased peak L-type Ca 2+ current and interstitial fibrosis in atria. The combined electrophysiological and structural changes facilitated the development of local conduction block and altered action potential duration and spontaneous AF. NEW & NOTEWORTHY This study addresses a long-standing riddle regarding the role of FK506-binding protein 12 in cardiac physiology. The work provides further evidence that FK506-binding protein 12 is a critical component for regulating voltage-gated sodium current and in so doing has an important role in arrhythmogenic physiology, such as atrial fibrillation.

KW - Action potential duration

KW - Cardiac electrophysiology

KW - Fibrosis

KW - Ion channels

KW - Patch clamp

KW - Voltage-gated sodium current

UR - http://www.scopus.com/inward/record.url?scp=85060916878&partnerID=8YFLogxK

U2 - 10.1152/ajpheart.00486.2018

DO - 10.1152/ajpheart.00486.2018

M3 - Article

VL - 316

SP - H371-H379

JO - American Journal of Physiology - Heart and Circulatory Physiology

JF - American Journal of Physiology - Heart and Circulatory Physiology

SN - 0363-6135

IS - 2

ER -