Assessment of aqueous humor dynamics in the rodent by constant flow infusion

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations

Abstract

Mice and rats are being increasingly used in glaucoma research and much useful data have been generated from them. One aspect of using these animals for this purpose involves assessment of aqueous humor dynamics. Several techniques have been described in the literature for the determination of one or more of these parameters in rodents, in both living animals and eyes perfused ex vivo. Here, we describe the practical details for a technique for the determination of all principal parameters of aqueous humor dynamics (intraocular pressure (IOP), aqueous humor formation rate (Fin), uveoscleral outflow rate (Fu), aqueous outflow facility (C), and episcleral venous pressure (Pe)) in the living rat and mouse eye, in a single experimental session.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages109-120
Number of pages12
DOIs
Publication statusPublished - 1 Jan 2018

Publication series

NameMethods in Molecular Biology
Volume1695
ISSN (Print)1064-3745

    Fingerprint

Keywords

  • Aqueous humor dynamics
  • Aqueous humor formation
  • Aqueous outflow facility
  • Episcleral venous pressure
  • Intraocular pressure
  • Mouse
  • Rat
  • Uveoscleral outflow

Cite this

Millar, J. C., Phan, T. N., & Pang, I. H. (2018). Assessment of aqueous humor dynamics in the rodent by constant flow infusion. In Methods in Molecular Biology (pp. 109-120). (Methods in Molecular Biology; Vol. 1695). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-7407-8_11