Assessment of aqueous humor dynamics in the rodent by constant flow infusion

J. Cameron Millar, Tien N. Phan, Iok Hou Pang

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

6 Scopus citations


Mice and rats are being increasingly used in glaucoma research and much useful data have been generated from them. One aspect of using these animals for this purpose involves assessment of aqueous humor dynamics. Several techniques have been described in the literature for the determination of one or more of these parameters in rodents, in both living animals and eyes perfused ex vivo. Here, we describe the practical details for a technique for the determination of all principal parameters of aqueous humor dynamics (intraocular pressure (IOP), aqueous humor formation rate (Fin), uveoscleral outflow rate (Fu), aqueous outflow facility (C), and episcleral venous pressure (Pe)) in the living rat and mouse eye, in a single experimental session.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages12
StatePublished - 1 Jan 2018

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745


  • Aqueous humor dynamics
  • Aqueous humor formation
  • Aqueous outflow facility
  • Episcleral venous pressure
  • Intraocular pressure
  • Mouse
  • Rat
  • Uveoscleral outflow


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