TY - JOUR
T1 - Applied Biosystems VeriFiler Plus PCR Amplification Kit with internal quality control system provides confident answers with challenging forensic samples
AU - Zhong, Chang
AU - Green, Robert
AU - Norona, Wilma
AU - Mulero, Julio
AU - Eardley, Rob
AU - Ge, Jianye
AU - Lackey, Angie
N1 - Publisher Copyright:
© 2019 Elsevier B.V.
PY - 2019/12
Y1 - 2019/12
N2 - The Applied Biosystems™ VeriFiler™ Plus PCR Amplification Kit has been developed to meet challenging forensic casework needs globally. It is a companion kit to the Applied Biosystems™ VeriFiler™ Express PCR Amplification Kit for databasing. Powered by a six-dye short tandem repeat (STR) chemistry and an improved master mix formulation, this kit is specifically designed for challenging sample types—touch, degraded, or inhibited samples with improved sensitivity and enhanced robustness against inhibition. With an recommended input of 0.5 ng, the VeriFiler Plus kit targets a total of 25 loci- 23 autosomal STRs, including the core CODIS loci, Penta D, Penta E and D6S1043, 2 gender discrimination markers, while also including an internal quality control (IQC) system. The IQC system contained in the VeriFiler Plus kit consists of two synthetic sequences with specific primers for each of the targets. This system provides positive confirmation that all assay components are functioning as expected. This system is particularly useful for confirming the validity of negative results and distinguishing samples that are degraded from those that contain PCR inhibitors.
AB - The Applied Biosystems™ VeriFiler™ Plus PCR Amplification Kit has been developed to meet challenging forensic casework needs globally. It is a companion kit to the Applied Biosystems™ VeriFiler™ Express PCR Amplification Kit for databasing. Powered by a six-dye short tandem repeat (STR) chemistry and an improved master mix formulation, this kit is specifically designed for challenging sample types—touch, degraded, or inhibited samples with improved sensitivity and enhanced robustness against inhibition. With an recommended input of 0.5 ng, the VeriFiler Plus kit targets a total of 25 loci- 23 autosomal STRs, including the core CODIS loci, Penta D, Penta E and D6S1043, 2 gender discrimination markers, while also including an internal quality control (IQC) system. The IQC system contained in the VeriFiler Plus kit consists of two synthetic sequences with specific primers for each of the targets. This system provides positive confirmation that all assay components are functioning as expected. This system is particularly useful for confirming the validity of negative results and distinguishing samples that are degraded from those that contain PCR inhibitors.
KW - Internal quality control system
KW - Multiplex STR PCR system
KW - VeriFiler Plus PCR Amplification kit
UR - http://www.scopus.com/inward/record.url?scp=85073760019&partnerID=8YFLogxK
U2 - 10.1016/j.fsigss.2019.10.044
DO - 10.1016/j.fsigss.2019.10.044
M3 - Article
AN - SCOPUS:85073760019
SN - 1875-1768
VL - 7
SP - 441
EP - 443
JO - Forensic Science International: Genetics Supplement Series
JF - Forensic Science International: Genetics Supplement Series
IS - 1
ER -