TY - CHAP
T1 - Application of mass spectrometry in brain research. neurotransmitters, neuropeptides, and brain proteins
AU - Prokai, Laszlo
N1 - Funding Information:
The author thanks Drs. Alevtina D. Zharikova, Stanley M. Stevens, Jr., and Ho-Seung Kim for contributing to the work described in this chapter, and Drs. Jerry W. Simecka and Katalin Prokai-Tatrai for their helpful comments on the manuscript. The studies on neuropeptides benefited from the author's collaboration with Drs. John Roboz and William J. Simonsick, Jr. The research presented here has been supported by grants (MH59360, NS44765, AG025384 and RR12023) from the National Institutes of Health (Bethesda, MD, USA) and the University of Florida Research Foundation. Laszlo Prokai is currently the Robert A. Welch Professor at the University of North Texas Health Science Center.
PY - 2008
Y1 - 2008
N2 - This chapter covers the selected applications of mass spectrometry and highlights its power to support diverse studies focusing on the mammalian brain. Microdialysis employs a semipermeable hollow-fiber membrane implanted in the tissue. It allows for the sampling of chemicals from the extracellular space of the brain, when the implanted probe is perfused at low flow rates and usually with a solution mimicking the composition of the cerebrospinal fluid (CSF). The reversed-phase ion-pair liquid chromatography (LC) coupled with positive-ion electrospray ionization (ESI) tandem mass spectrometry (MS/MS) has been shown to detect ACh with low limit of detection (1.4 fmol) and, thus, to measure this neurotransmitter and related endogenous compounds in rat brain microdialysates. Neuropeptides collected by microdialysis can be preconcentrated and desalted by reversed-phase LC, and subsequently supplied directly onto a micro- or nanoflow-LC column for gradient elution, and ESI-MS as well as MS/MS analysis. The separation and visualization of complex protein mixtures are commonly performed by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE). 2D-PAGE followed by in-gel protease (trypsin) digestion, matrix-assisted laser desorption/ionization (MALDI)/time-of-flight (TOF) mass spectrometry, and sequence database searching is the technique most frequently used in today's neuroproteomics studies.
AB - This chapter covers the selected applications of mass spectrometry and highlights its power to support diverse studies focusing on the mammalian brain. Microdialysis employs a semipermeable hollow-fiber membrane implanted in the tissue. It allows for the sampling of chemicals from the extracellular space of the brain, when the implanted probe is perfused at low flow rates and usually with a solution mimicking the composition of the cerebrospinal fluid (CSF). The reversed-phase ion-pair liquid chromatography (LC) coupled with positive-ion electrospray ionization (ESI) tandem mass spectrometry (MS/MS) has been shown to detect ACh with low limit of detection (1.4 fmol) and, thus, to measure this neurotransmitter and related endogenous compounds in rat brain microdialysates. Neuropeptides collected by microdialysis can be preconcentrated and desalted by reversed-phase LC, and subsequently supplied directly onto a micro- or nanoflow-LC column for gradient elution, and ESI-MS as well as MS/MS analysis. The separation and visualization of complex protein mixtures are commonly performed by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE). 2D-PAGE followed by in-gel protease (trypsin) digestion, matrix-assisted laser desorption/ionization (MALDI)/time-of-flight (TOF) mass spectrometry, and sequence database searching is the technique most frequently used in today's neuroproteomics studies.
UR - http://www.scopus.com/inward/record.url?scp=84882835398&partnerID=8YFLogxK
U2 - 10.1016/B978-044451980-1.50020-3
DO - 10.1016/B978-044451980-1.50020-3
M3 - Chapter
AN - SCOPUS:84882835398
SN - 9780444519801
SP - 407
EP - 424
BT - Medical Applications of Mass Spectrometry
PB - Elsevier
ER -