Apolipoprotein D in the aging brain and in Alzheimer's dementia

Janos Kalman, Walter McConathy, Carlos Araoz, Peter Kasa, Andras G. Lacko

Research output: Contribution to journalArticle

70 Citations (Scopus)

Abstract

Apolipoprotein D (apoD) levels were examined in the temporal cortex as well as an assessment of the location of apoD positive cells within the brain by immunohistochemical and biochemical methods in young control (YC), aged control (AC), and Alzheimer's demented (AD) probands. Scattered apoD positive astrocytes and oligodendrocytes were found throughout the white matter by immunohistochemistry. ApoD immunoreactivity was also observed in the cerebellar oligodendrocytes of the YC group. There was faint positive apoD staining in scattered cortical astrocytes and a few neurons in the same group. In contrast, some of the AC and all of the AD probands had intense and frequent apoD immunostained cortical astrocytes and pyramidal neurons. The cortical senile plaques and neurofibrillary tangles were apoD immunonegative. No quantitative differences were found between the cortical apoD levels in the AC and AD groups, determined by immunoblotting. ApoD detected in the brain tissue was different in molecular weight (29 kDal) from that seen in CSF or in the serum (32 kDal). Our results indicate apoD is present in the human brain, especially in glial cells, and has increased abundance in the elderly and AD subjects.

Original languageEnglish
Pages (from-to)330-336
Number of pages7
JournalNeurological Research
Volume22
Issue number4
DOIs
StatePublished - 1 Jan 2000

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Apolipoproteins D
Alzheimer Disease
Brain
Astrocytes
Oligodendroglia
Neurofibrillary Tangles
Pyramidal Cells
Amyloid Plaques
Temporal Lobe
Immunoblotting
Neuroglia
Molecular Weight
Immunohistochemistry
Staining and Labeling
Neurons

Keywords

  • Aging
  • Alzheimer's dementia
  • Apolipoprotein D
  • Brain

Cite this

Kalman, Janos ; McConathy, Walter ; Araoz, Carlos ; Kasa, Peter ; Lacko, Andras G. / Apolipoprotein D in the aging brain and in Alzheimer's dementia. In: Neurological Research. 2000 ; Vol. 22, No. 4. pp. 330-336.
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Apolipoprotein D in the aging brain and in Alzheimer's dementia. / Kalman, Janos; McConathy, Walter; Araoz, Carlos; Kasa, Peter; Lacko, Andras G.

In: Neurological Research, Vol. 22, No. 4, 01.01.2000, p. 330-336.

Research output: Contribution to journalArticle

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