TY - JOUR
T1 - Anterior segment physiology after bumetanide inhibition of Na-K-Cl cotransport
AU - Gabelt, B'Ann True
AU - Wiederholt, Michael
AU - Clark, Abbot F.
AU - Kaufman, Paul L.
PY - 1997/8
Y1 - 1997/8
N2 - Purpose. To determine the effect of bumetanide inhibition of Na-K-Cl cotransport on aqueous humor formation and outflow facility in living cynomolgus monkeys, outflow facility in organ-cultured human eyes, and contraction in bovine ciliary muscle and trabecular meshwork strips, in vitro. Methods. Aqueous humor formation in monkeys was measured fluorophotometrically for 6 hours, 1 to S weeks before, immediately after, and 2 to 6 weeks after bumetanide was administered intravitreally (final concentration ~100 or 500 μM) or intravenously (0.01 or 0.03 mg/kg at 0 and 3 hours). Outflow facility in monkeys was determined by two-level, constant- pressure perfusion of the anterior chamber for 45 to 60 minutes before and after bumetanide was administered by bolus intracameral injection (100 μM, initial anterior chamber concentration) or by exchanging the anterior chamber with 2 ml 10, 100, or 500 μM bumetanide. Urine volume was measured S hours after administration of intravenous bumetanide in various diluents. The effect on intraocular pressure in organ-cultured human eyes was determined for 48 hours by constant-flow-variable-pressure perfusion with 10 μM bumetanide. Contraction of fresh bovine ciliary muscle and trabecular meshwork was measured isometrically with a force-length transducer system after exposure to 100 μM bumetanide ± 1 μM carbachol. Results. The bumetanide concentrations used had little effect on outflow facility or on aqueous humor formation in normal monkeys, on intraocular pressure in organ- cultured human eyes, or on contraction of bovine ciliary muscle and trabecular meshwork strips. Intravenous bumetanide increased urine volume, regardless of the diluent used. Conclusions. These results suggest that Na- K-Cl cotransport is not involved functionally in regulation of aqueous humor inflow and outflow and in contractility of ciliary muscle and trabecular meshwork.
AB - Purpose. To determine the effect of bumetanide inhibition of Na-K-Cl cotransport on aqueous humor formation and outflow facility in living cynomolgus monkeys, outflow facility in organ-cultured human eyes, and contraction in bovine ciliary muscle and trabecular meshwork strips, in vitro. Methods. Aqueous humor formation in monkeys was measured fluorophotometrically for 6 hours, 1 to S weeks before, immediately after, and 2 to 6 weeks after bumetanide was administered intravitreally (final concentration ~100 or 500 μM) or intravenously (0.01 or 0.03 mg/kg at 0 and 3 hours). Outflow facility in monkeys was determined by two-level, constant- pressure perfusion of the anterior chamber for 45 to 60 minutes before and after bumetanide was administered by bolus intracameral injection (100 μM, initial anterior chamber concentration) or by exchanging the anterior chamber with 2 ml 10, 100, or 500 μM bumetanide. Urine volume was measured S hours after administration of intravenous bumetanide in various diluents. The effect on intraocular pressure in organ-cultured human eyes was determined for 48 hours by constant-flow-variable-pressure perfusion with 10 μM bumetanide. Contraction of fresh bovine ciliary muscle and trabecular meshwork was measured isometrically with a force-length transducer system after exposure to 100 μM bumetanide ± 1 μM carbachol. Results. The bumetanide concentrations used had little effect on outflow facility or on aqueous humor formation in normal monkeys, on intraocular pressure in organ- cultured human eyes, or on contraction of bovine ciliary muscle and trabecular meshwork strips. Intravenous bumetanide increased urine volume, regardless of the diluent used. Conclusions. These results suggest that Na- K-Cl cotransport is not involved functionally in regulation of aqueous humor inflow and outflow and in contractility of ciliary muscle and trabecular meshwork.
KW - Aqueous humor dynamics
KW - Bumetanide
KW - Ciliary muscle
KW - Na-K-Cl cotransport
KW - Trabecular meshwork
UR - http://www.scopus.com/inward/record.url?scp=0030808502&partnerID=8YFLogxK
M3 - Article
C2 - 9286258
AN - SCOPUS:0030808502
SN - 0146-0404
VL - 38
SP - 1700
EP - 1707
JO - Investigative Ophthalmology and Visual Science
JF - Investigative Ophthalmology and Visual Science
IS - 9
ER -