Anisotropy spectra of indole and N-acetyl-L-tryptophanamide observed for two-photon excitation of fluorescence

Joseph R. Lakowicz, Ignacy Gryczynski, Eva Danielsen, Joan Frisoli

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48 Scopus citations

Abstract

We observed the steady state fluorescence anisotropy spectra of indole and N-acetyl-L-tryptophanamide (NATA) resulting from one- (OPE) and two-photon excitation (TPE) of fluorescence. The anisotropy spectra for OPE and TPE are remarkably different, suggesting that the cross sections for relative absorption of the 1La and 1Lb state are different for one- and two-photon absorption. Frequency-domain time-resolved anisotropy decays confirm the unusual value of the time-zero anisotropies. Comparison of the OPE and TPE anisotropy spectra should allow a more critical evaluation of the accepted 1La-1Lb model for tryptophan and protein fluorescence.

Original languageEnglish
Pages (from-to)282-287
Number of pages6
JournalChemical Physics Letters
Volume194
Issue number4-6
DOIs
StatePublished - 3 Jul 1992

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