Anisotropy spectra of indole and N-acetyl-L-tryptophanamide observed for two-photon excitation of fluorescence

Joseph R. Lakowicz; Ignacy Gryczynski; Eva Danielsen; Joan Frisoli

doi:10.1016/0009-2614(92)86052-J

Anisotropy spectra of indole and N-acetyl-L-tryptophanamide observed for two-photon excitation of fluorescence

Joseph R. Lakowicz, Ignacy Gryczynski, Eva Danielsen, Joan Frisoli

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Abstract

We observed the steady state fluorescence anisotropy spectra of indole and N-acetyl-L-tryptophanamide (NATA) resulting from one- (OPE) and two-photon excitation (TPE) of fluorescence. The anisotropy spectra for OPE and TPE are remarkably different, suggesting that the cross sections for relative absorption of the ¹L_a and ¹L_b state are different for one- and two-photon absorption. Frequency-domain time-resolved anisotropy decays confirm the unusual value of the time-zero anisotropies. Comparison of the OPE and TPE anisotropy spectra should allow a more critical evaluation of the accepted ¹L_a-¹L_b model for tryptophan and protein fluorescence.

Original language	English
Pages (from-to)	282-287
Number of pages	6
Journal	Chemical Physics Letters
Volume	194
Issue number	4-6
DOIs	https://doi.org/10.1016/0009-2614(92)86052-J
State	Published - 3 Jul 1992

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@article{771909757fd649efa1604f8f172f058b,

title = "Anisotropy spectra of indole and N-acetyl-L-tryptophanamide observed for two-photon excitation of fluorescence",

abstract = "We observed the steady state fluorescence anisotropy spectra of indole and N-acetyl-L-tryptophanamide (NATA) resulting from one- (OPE) and two-photon excitation (TPE) of fluorescence. The anisotropy spectra for OPE and TPE are remarkably different, suggesting that the cross sections for relative absorption of the 1La and 1Lb state are different for one- and two-photon absorption. Frequency-domain time-resolved anisotropy decays confirm the unusual value of the time-zero anisotropies. Comparison of the OPE and TPE anisotropy spectra should allow a more critical evaluation of the accepted 1La-1Lb model for tryptophan and protein fluorescence.",

author = "Lakowicz, {Joseph R.} and Ignacy Gryczynski and Eva Danielsen and Joan Frisoli",

note = "Funding Information: The authors acknowledge support from the National ScienceF oundation (DIR-87 10401 and DMB-8804931), the National Institutes of Health (RR-04800 and RR-07510) and the Medical Biotechnology Center and Graduate School at the University of Maryland. ED was on sabbatical leave from the Royal Veterinary and Agricultural University, Copenhagena nd also a recipient of a NATO Science Fellowship.",

year = "1992",

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day = "3",

doi = "10.1016/0009-2614(92)86052-J",

language = "English",

volume = "194",

pages = "282--287",

journal = "Chemical Physics Letters",

issn = "0009-2614",

publisher = "Elsevier",

number = "4-6",

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TY - JOUR

T1 - Anisotropy spectra of indole and N-acetyl-L-tryptophanamide observed for two-photon excitation of fluorescence

AU - Lakowicz, Joseph R.

AU - Gryczynski, Ignacy

AU - Danielsen, Eva

AU - Frisoli, Joan

N1 - Funding Information: The authors acknowledge support from the National ScienceF oundation (DIR-87 10401 and DMB-8804931), the National Institutes of Health (RR-04800 and RR-07510) and the Medical Biotechnology Center and Graduate School at the University of Maryland. ED was on sabbatical leave from the Royal Veterinary and Agricultural University, Copenhagena nd also a recipient of a NATO Science Fellowship.

PY - 1992/7/3

Y1 - 1992/7/3

N2 - We observed the steady state fluorescence anisotropy spectra of indole and N-acetyl-L-tryptophanamide (NATA) resulting from one- (OPE) and two-photon excitation (TPE) of fluorescence. The anisotropy spectra for OPE and TPE are remarkably different, suggesting that the cross sections for relative absorption of the 1La and 1Lb state are different for one- and two-photon absorption. Frequency-domain time-resolved anisotropy decays confirm the unusual value of the time-zero anisotropies. Comparison of the OPE and TPE anisotropy spectra should allow a more critical evaluation of the accepted 1La-1Lb model for tryptophan and protein fluorescence.

AB - We observed the steady state fluorescence anisotropy spectra of indole and N-acetyl-L-tryptophanamide (NATA) resulting from one- (OPE) and two-photon excitation (TPE) of fluorescence. The anisotropy spectra for OPE and TPE are remarkably different, suggesting that the cross sections for relative absorption of the 1La and 1Lb state are different for one- and two-photon absorption. Frequency-domain time-resolved anisotropy decays confirm the unusual value of the time-zero anisotropies. Comparison of the OPE and TPE anisotropy spectra should allow a more critical evaluation of the accepted 1La-1Lb model for tryptophan and protein fluorescence.

UR - http://www.scopus.com/inward/record.url?scp=0000807722&partnerID=8YFLogxK

U2 - 10.1016/0009-2614(92)86052-J

DO - 10.1016/0009-2614(92)86052-J

M3 - Article

AN - SCOPUS:0000807722

VL - 194

SP - 282

EP - 287

JO - Chemical Physics Letters

JF - Chemical Physics Letters

SN - 0009-2614

IS - 4-6

ER -

Anisotropy spectra of indole and N-acetyl-L-tryptophanamide observed for two-photon excitation of fluorescence

Abstract

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