TY - JOUR
T1 - ANISOTROPY DECAYS of SINGLE TRYPTOPHAN PROTEINS MEASURED by GHz FREQUENCY-DOMAIN FLUOROMETRY with COLLISIONAL QUENCHING
AU - Lakowicz, Joseph R.
AU - Gryczynski, Ignacy
AU - Szmacinski, Henryk
AU - Cherek, Henryk
AU - Joshi, Nanda
N1 - Publisher Copyright:
© 1988 SPIE. All rights reserved.
PY - 1988/6/24
Y1 - 1988/6/24
N2 - We use frequency-domain fluorometry to determine the anisotropy decays of the tryptophan emission from S. nuclease and from the model compound gly-trp-gly. Resolution of the rapid and complex anisotropy decays was enhanced by global analysis of the data measured in the presence of quenching by either oxygen or acrylamide. Data were obtained at four to six quencher concentrations, and the data analyzed globally to recover the anisotropy decay. Because the decay times were decreased by quenching, measurements were possible to a upper frequency limit of 2 GHz. The anisotropy decay of gly-trp-gly revealed 40 ps of the indole ring, which was resolved from the overall 150 ps correlation times of the tripeptide. The anisotropy decay of nuclease displayed a 90 /ps component as well as a 10 ns component due to overall rotational diffusion. We believe these highly resolved anisotropy decays are suitably for comparison with molecular dynamic simulations.
AB - We use frequency-domain fluorometry to determine the anisotropy decays of the tryptophan emission from S. nuclease and from the model compound gly-trp-gly. Resolution of the rapid and complex anisotropy decays was enhanced by global analysis of the data measured in the presence of quenching by either oxygen or acrylamide. Data were obtained at four to six quencher concentrations, and the data analyzed globally to recover the anisotropy decay. Because the decay times were decreased by quenching, measurements were possible to a upper frequency limit of 2 GHz. The anisotropy decay of gly-trp-gly revealed 40 ps of the indole ring, which was resolved from the overall 150 ps correlation times of the tripeptide. The anisotropy decay of nuclease displayed a 90 /ps component as well as a 10 ns component due to overall rotational diffusion. We believe these highly resolved anisotropy decays are suitably for comparison with molecular dynamic simulations.
UR - http://www.scopus.com/inward/record.url?scp=84913484403&partnerID=8YFLogxK
U2 - 10.1117/12.945384
DO - 10.1117/12.945384
M3 - Conference article
AN - SCOPUS:84913484403
SN - 0277-786X
VL - 909
SP - 178
EP - 185
JO - Proceedings of SPIE - The International Society for Optical Engineering
JF - Proceedings of SPIE - The International Society for Optical Engineering
T2 - Time-Resolved Laser Spectroscopy in Biochemistry 1988
Y2 - 11 January 1988 through 17 January 1988
ER -