TY - JOUR
T1 - Anisotropic rotational diffusion of indole in cyclohexane studied by 2 GHz frequency-domain fluorometry
AU - Lakowicz, Joseph R.
AU - Gryczynski, Ignacy
AU - Wiczk, Wieslaw M.
N1 - Funding Information:
Supportedb y Grants DMB-851 1065a nd DMB-8502835fr om theN ationalS cienceF oundationa nd Grant GM-39617 from the National Instituteso f Health.
PY - 1988/8/12
Y1 - 1988/8/12
N2 - We measured the frequency response of the polarized emission of non-quenched and quenched indole in cyclohexane at 20°C, up to 2 GHz. Non-radiative energy transfer from indole to t-stilbene was used to decrease indole decay time. Quenching increases the fraction of the total emission, which occurs on a picosecond timescale, and thereby provides increased information on rapid rotational motions. The measurement of quenched sample allowed resolution of the anisotropic rotation of indole with correlation times of 17 and 73 ps. The longer correlation time is consistent with that expected for a sphere with stick boundary conditions, the shorter with the slip boundary condition for an oblate ellipsoid.
AB - We measured the frequency response of the polarized emission of non-quenched and quenched indole in cyclohexane at 20°C, up to 2 GHz. Non-radiative energy transfer from indole to t-stilbene was used to decrease indole decay time. Quenching increases the fraction of the total emission, which occurs on a picosecond timescale, and thereby provides increased information on rapid rotational motions. The measurement of quenched sample allowed resolution of the anisotropic rotation of indole with correlation times of 17 and 73 ps. The longer correlation time is consistent with that expected for a sphere with stick boundary conditions, the shorter with the slip boundary condition for an oblate ellipsoid.
UR - http://www.scopus.com/inward/record.url?scp=0041044378&partnerID=8YFLogxK
U2 - 10.1016/0009-2614(88)87209-9
DO - 10.1016/0009-2614(88)87209-9
M3 - Article
AN - SCOPUS:0041044378
SN - 0009-2614
VL - 149
SP - 134
EP - 139
JO - Chemical Physics Letters
JF - Chemical Physics Letters
IS - 2
ER -