Angiotensin II has acute effects on TRPC6 channels in podocytes of freshly isolated glomeruli

Daria V. Ilatovskaya, Oleg Palygin, Vladislav Chubinskiy-Nadezhdin, Yuri A. Negulyaev, Rong Ma, Lutz Birnbaumer, Alexander Staruschenko

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

A key role for podocytes in the pathogenesis of proteinuric renal diseases has been established. Angiotensin II causes depolarization and increased intracellular calcium concentration in podocytes; members of the cation TRPC channels family, particularly TRPC6, are proposed as proteins responsible for calcium flux. Angiotensin II evokes calcium transient through TRPC channels and mutations in the gene encoding the TRPC6 channel result in the development of focal segmental glomerulosclerosis. Here we examined the effects of angiotensin II on intracellular calcium ion levels and endogenous channels in intact podocytes of freshly isolated decapsulated mouse glomeruli. An ion channel with distinct TRPC6 properties was identified in wild-type, but was absent in TRPC6 knockout mice. Single-channel electrophysiological analysis found that angiotensin II acutely activated native TRPC-like channels in both podocytes of freshly isolated glomeruli and TRPC6 channels transiently overexpressed in CHO cells; the effect was mediated by changes in the channel open probability. Angiotensin II evoked intracellular calcium transients in the wild-type podocytes, which was blunted in TRPC6 knockout glomeruli. Pan-TRPC inhibitors gadolinium and SKF 96365 reduced the response in wild-type glomerular epithelial cells, whereas the transient in TRPC6 knockout animals was not affected. Thus, angiotensin II-dependent activation of TRPC6 channels in podocytes may have a significant role in the development of kidney diseases.

Original languageEnglish
Pages (from-to)506-514
Number of pages9
JournalKidney International
Volume86
Issue number3
DOIs
StatePublished - Sep 2014

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Podocytes
Angiotensin II
Calcium
1-(2-(3-(4-methoxyphenyl)propoxy)-4-methoxyphenylethyl)-1H-imidazole
TRPC Cation Channels
Focal Segmental Glomerulosclerosis
CHO Cells
Gadolinium
Kidney Diseases
Ion Channels
Knockout Mice
Epithelial Cells
Ions
Kidney
Mutation
Genes
Proteins

Keywords

  • angiotensin
  • calcium
  • focal segmental glomerulosclerosis
  • ion channel
  • nephrotic syndrome
  • podocyte

Cite this

Ilatovskaya, D. V., Palygin, O., Chubinskiy-Nadezhdin, V., Negulyaev, Y. A., Ma, R., Birnbaumer, L., & Staruschenko, A. (2014). Angiotensin II has acute effects on TRPC6 channels in podocytes of freshly isolated glomeruli. Kidney International, 86(3), 506-514. https://doi.org/10.1038/ki.2014.71
Ilatovskaya, Daria V. ; Palygin, Oleg ; Chubinskiy-Nadezhdin, Vladislav ; Negulyaev, Yuri A. ; Ma, Rong ; Birnbaumer, Lutz ; Staruschenko, Alexander. / Angiotensin II has acute effects on TRPC6 channels in podocytes of freshly isolated glomeruli. In: Kidney International. 2014 ; Vol. 86, No. 3. pp. 506-514.
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Ilatovskaya, DV, Palygin, O, Chubinskiy-Nadezhdin, V, Negulyaev, YA, Ma, R, Birnbaumer, L & Staruschenko, A 2014, 'Angiotensin II has acute effects on TRPC6 channels in podocytes of freshly isolated glomeruli', Kidney International, vol. 86, no. 3, pp. 506-514. https://doi.org/10.1038/ki.2014.71

Angiotensin II has acute effects on TRPC6 channels in podocytes of freshly isolated glomeruli. / Ilatovskaya, Daria V.; Palygin, Oleg; Chubinskiy-Nadezhdin, Vladislav; Negulyaev, Yuri A.; Ma, Rong; Birnbaumer, Lutz; Staruschenko, Alexander.

In: Kidney International, Vol. 86, No. 3, 09.2014, p. 506-514.

Research output: Contribution to journalArticle

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T1 - Angiotensin II has acute effects on TRPC6 channels in podocytes of freshly isolated glomeruli

AU - Ilatovskaya, Daria V.

AU - Palygin, Oleg

AU - Chubinskiy-Nadezhdin, Vladislav

AU - Negulyaev, Yuri A.

AU - Ma, Rong

AU - Birnbaumer, Lutz

AU - Staruschenko, Alexander

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AB - A key role for podocytes in the pathogenesis of proteinuric renal diseases has been established. Angiotensin II causes depolarization and increased intracellular calcium concentration in podocytes; members of the cation TRPC channels family, particularly TRPC6, are proposed as proteins responsible for calcium flux. Angiotensin II evokes calcium transient through TRPC channels and mutations in the gene encoding the TRPC6 channel result in the development of focal segmental glomerulosclerosis. Here we examined the effects of angiotensin II on intracellular calcium ion levels and endogenous channels in intact podocytes of freshly isolated decapsulated mouse glomeruli. An ion channel with distinct TRPC6 properties was identified in wild-type, but was absent in TRPC6 knockout mice. Single-channel electrophysiological analysis found that angiotensin II acutely activated native TRPC-like channels in both podocytes of freshly isolated glomeruli and TRPC6 channels transiently overexpressed in CHO cells; the effect was mediated by changes in the channel open probability. Angiotensin II evoked intracellular calcium transients in the wild-type podocytes, which was blunted in TRPC6 knockout glomeruli. Pan-TRPC inhibitors gadolinium and SKF 96365 reduced the response in wild-type glomerular epithelial cells, whereas the transient in TRPC6 knockout animals was not affected. Thus, angiotensin II-dependent activation of TRPC6 channels in podocytes may have a significant role in the development of kidney diseases.

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KW - nephrotic syndrome

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Ilatovskaya DV, Palygin O, Chubinskiy-Nadezhdin V, Negulyaev YA, Ma R, Birnbaumer L et al. Angiotensin II has acute effects on TRPC6 channels in podocytes of freshly isolated glomeruli. Kidney International. 2014 Sep;86(3):506-514. https://doi.org/10.1038/ki.2014.71