Analysis of short tandem repeat (STR) HUMVWA in the Spanish population

Jose A. Lorente; Miguel Lorente; Bruce Budowle; Mark R. Wilson; Enrique Villanueva

doi:10.1016/0379-0738(94)90272-0

Analysis of short tandem repeat (STR) HUMVWA in the Spanish population

Jose A. Lorente, Miguel Lorente, Bruce Budowle, Mark R. Wilson, Enrique Villanueva

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

Amplification by polymerase chain reaction (PCR) of variable number of tandem repeat (VNTR) loci and subsequent typing by electrophoresis and silver staining has become a useful tool for identity testing. One viable group of genetic markers amenable to amplification by PCR is the short tandem repeat (STR) loci. A horizontal discontinuous polyacrylamide gel electrophoresis (PAGE) method was used to type the amplified products of the STR HUMVWA. Typing for VWA of 120 unrelated Spanish Caucasians was done. Six alleles were observed with frequencies in the range 0.096-0.242. The genotype distribution meets Hardy-Weinberg expectations (0.25 < P < 0.50). The heterozygosity was 73.3% and the discrimination power (DP) 0.94. Simultaneously, in a small sample of families (n = 24) no new mutations could be found.

Original language	English
Pages (from-to)	169-175
Number of pages	7
Journal	Forensic Science International
Volume	65
Issue number	3
DOIs	https://doi.org/10.1016/0379-0738(94)90272-0
State	Published - 13 May 1994

Keywords

Forensic identity
PCR
Population genetics
STR
VNTR

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10.1016/0379-0738(94)90272-0

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title = "Analysis of short tandem repeat (STR) HUMVWA in the Spanish population",

abstract = "Amplification by polymerase chain reaction (PCR) of variable number of tandem repeat (VNTR) loci and subsequent typing by electrophoresis and silver staining has become a useful tool for identity testing. One viable group of genetic markers amenable to amplification by PCR is the short tandem repeat (STR) loci. A horizontal discontinuous polyacrylamide gel electrophoresis (PAGE) method was used to type the amplified products of the STR HUMVWA. Typing for VWA of 120 unrelated Spanish Caucasians was done. Six alleles were observed with frequencies in the range 0.096-0.242. The genotype distribution meets Hardy-Weinberg expectations (0.25 < P < 0.50). The heterozygosity was 73.3% and the discrimination power (DP) 0.94. Simultaneously, in a small sample of families (n = 24) no new mutations could be found.",

keywords = "Forensic identity, PCR, Population genetics, STR, VNTR",

author = "Lorente, {Jose A.} and Miguel Lorente and Bruce Budowle and Wilson, {Mark R.} and Enrique Villanueva",

note = "Funding Information: This work was partially supported by a 1991 grant from the Scientific Committee of NATO to J.A. Lorente and by a 1992 grant from the Junta de Andalucia to M. Lorente.",

year = "1994",

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doi = "10.1016/0379-0738(94)90272-0",

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journal = "Forensic Science International",

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T1 - Analysis of short tandem repeat (STR) HUMVWA in the Spanish population

AU - Lorente, Jose A.

AU - Lorente, Miguel

AU - Budowle, Bruce

AU - Wilson, Mark R.

AU - Villanueva, Enrique

N1 - Funding Information: This work was partially supported by a 1991 grant from the Scientific Committee of NATO to J.A. Lorente and by a 1992 grant from the Junta de Andalucia to M. Lorente.

PY - 1994/5/13

Y1 - 1994/5/13

N2 - Amplification by polymerase chain reaction (PCR) of variable number of tandem repeat (VNTR) loci and subsequent typing by electrophoresis and silver staining has become a useful tool for identity testing. One viable group of genetic markers amenable to amplification by PCR is the short tandem repeat (STR) loci. A horizontal discontinuous polyacrylamide gel electrophoresis (PAGE) method was used to type the amplified products of the STR HUMVWA. Typing for VWA of 120 unrelated Spanish Caucasians was done. Six alleles were observed with frequencies in the range 0.096-0.242. The genotype distribution meets Hardy-Weinberg expectations (0.25 < P < 0.50). The heterozygosity was 73.3% and the discrimination power (DP) 0.94. Simultaneously, in a small sample of families (n = 24) no new mutations could be found.

AB - Amplification by polymerase chain reaction (PCR) of variable number of tandem repeat (VNTR) loci and subsequent typing by electrophoresis and silver staining has become a useful tool for identity testing. One viable group of genetic markers amenable to amplification by PCR is the short tandem repeat (STR) loci. A horizontal discontinuous polyacrylamide gel electrophoresis (PAGE) method was used to type the amplified products of the STR HUMVWA. Typing for VWA of 120 unrelated Spanish Caucasians was done. Six alleles were observed with frequencies in the range 0.096-0.242. The genotype distribution meets Hardy-Weinberg expectations (0.25 < P < 0.50). The heterozygosity was 73.3% and the discrimination power (DP) 0.94. Simultaneously, in a small sample of families (n = 24) no new mutations could be found.

KW - Forensic identity

KW - PCR

KW - Population genetics

KW - STR

KW - VNTR

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U2 - 10.1016/0379-0738(94)90272-0

DO - 10.1016/0379-0738(94)90272-0

M3 - Article

C2 - 8039774

AN - SCOPUS:0028335407

SN - 0379-0738

VL - 65

SP - 169

EP - 175

JO - Forensic Science International

JF - Forensic Science International

IS - 3

ER -

Analysis of short tandem repeat (STR) HUMVWA in the Spanish population

Abstract

Keywords

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