Analysis of fetal rat liver using monoclonal antibodies

Rehana Sultana Lovely, Yu Hsueh Wang, Nobuko Tokuda, Tomoo Sawada, Yoshihisa Fujikura, Tetsuo Fukumoto

Research output: Contribution to journalArticle

Abstract

Background: In previous studies, we developed several monoclonal antibodies (mAbs) against fetal and adult rat liver to analyze the hemopoietic microenvironment of the fetal liver during the gestational period. In this study, we have developed two new mAbs against fetal rat liver cells and have examined the characteristics at various gestational ages of fetal liver and of adult liver. Methods: The characteristics of these monoclonal antibodies were demonstrated by examining several tissues using immunohistochemical staining and flow cytofluorometry. Results: Monoclonal antibodies HAM10 and HAM11 were developed against fetal rat liver cells. These reacted with the cytoplasm of fetal and adult hepatocytes. HAM10 antigen expression was strong at approximately day 18 of gestation in the active period of hemopoiesis in fetal rat liver but was much lower in adult liver. HAM10 antigen expression also increased in liver after partial hepatectomy and was reduced abruptly to a normal level thereafter. HAM11 antigen expression in fetal liver was weaker than that of HAM10 antigen expression. The degree of HAM11 antigen expression increased as gestation proceeded, reaching a maximum in adult liver. Conclusions: Both HAM10 and HAM11 antigens may play a role in the morphogenesis of hepatocytes and in the hemopoietic microenvironment for hemopoietic cells. Moreover, HAM10 antigen is may play a role in hepatocyte proliferation in the fetal liver, whereas HAM11 antigen may contribute to the maturation of fetal- to the adult-type hepatocytes.

Original languageEnglish
Pages (from-to)394-402
Number of pages9
JournalAnatomical Record
Volume246
Issue number3
DOIs
StatePublished - 7 Dec 1996

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Monoclonal Antibodies
Liver
Antigens
Hepatocytes
Cellular Microenvironment
Pregnancy
Hepatectomy
Morphogenesis
Gestational Age
Flow Cytometry
Cytoplasm
Staining and Labeling

Keywords

  • Fetal liver
  • Hemopoiesis
  • Hepatocytes
  • Monoclonal antibodies

Cite this

Lovely, Rehana Sultana ; Wang, Yu Hsueh ; Tokuda, Nobuko ; Sawada, Tomoo ; Fujikura, Yoshihisa ; Fukumoto, Tetsuo. / Analysis of fetal rat liver using monoclonal antibodies. In: Anatomical Record. 1996 ; Vol. 246, No. 3. pp. 394-402.
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Analysis of fetal rat liver using monoclonal antibodies. / Lovely, Rehana Sultana; Wang, Yu Hsueh; Tokuda, Nobuko; Sawada, Tomoo; Fujikura, Yoshihisa; Fukumoto, Tetsuo.

In: Anatomical Record, Vol. 246, No. 3, 07.12.1996, p. 394-402.

Research output: Contribution to journalArticle

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AU - Lovely, Rehana Sultana

AU - Wang, Yu Hsueh

AU - Tokuda, Nobuko

AU - Sawada, Tomoo

AU - Fujikura, Yoshihisa

AU - Fukumoto, Tetsuo

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