Analysis of DNA forensic markers using high throughput mass spectrometry

Steven A. Hofstadler, Thomas A. Hall, Kristin A. Sannes-Lowery, Sheri Manalili, Jessica E. Paulsen, Leslie D. McCurdy, Lora Gioeni, Thuy Penella, Arthur J. Eisenberg, John V. Planz, Bruce Budowle

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

A novel DNA forensics platform has been developed that is based on automated high throughput electrospray ionization mass spectrometry (ESI-MS). The approach uses ESI-MS to "weigh" DNA markers with enough accuracy to yield an unambiguous base composition (Aw, Gx, Cy, Tz) which are then used to derive a DNA profile for an individual. A number of blinded studies have been performed to evaluate the platform for both STR and mtDNA typing. The same platform is used for both analyses and offers advantages over the conventional typing approaches. This approach facilitates the analysis of mtDNA samples containing length heteroplasmy in the HV1/HV2 regions in situations that are not amenable to sequencing. Based on the base compositions profiles from a 24-primer pair assay which covers CRS 15924-16428 and 31-576, this approach is more resolving than traditional sequencing approaches which cover 16024-16365 and 73-340 and facilitates mixture analysis. STR markers can be analyzed in a similar fashion. The high mass accuracy of the measurements affords the ability to detect SNPs within the STR markers. A survey of samples from different population groups suggests that the frequency of SNPs in several of the CODIS loci is in excess of 30%; SNPs were identified in 10 of the 13 core loci.

Original languageEnglish
Pages (from-to)524-526
Number of pages3
JournalForensic Science International: Genetics Supplement Series
Volume2
Issue number1
DOIs
StatePublished - 1 Jan 2009

Keywords

  • Base composition
  • Heteroplasmy
  • Mass spectrometry
  • SNP
  • STR
  • mtDNA

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    Hofstadler, S. A., Hall, T. A., Sannes-Lowery, K. A., Manalili, S., Paulsen, J. E., McCurdy, L. D., Gioeni, L., Penella, T., Eisenberg, A. J., Planz, J. V., & Budowle, B. (2009). Analysis of DNA forensic markers using high throughput mass spectrometry. Forensic Science International: Genetics Supplement Series, 2(1), 524-526. https://doi.org/10.1016/j.fsigss.2009.08.140