An in vitro oxygen–glucose deprivation model for studying ischemia–reperfusion injury of neuronal cells

Myoung gwi Ryou, Robert T. Mallet

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Citations (Scopus)

Abstract

Ischemia–reperfusion syndromes of the heart and brain are the leading cause of death and long-term disability worldwide. Development of effective treatments for myocardial infarction, stroke, cardiac arrest and their sequelae requires preclinical models that replicate specific features of ischemia–reperfusion. The complexities of intact animals, including the integrated function of organ systems, autonomic innervation and endocrine factors, often preclude detailed study of specific components of ischemia–reperfusion injury cascades. Ischemia represents the interruption of metabolic fuel and oxygen delivery to support cellular oxidative metabolism; reintroduction of oxygen upon reperfusion of ischemic tissue triggers oxidative stress which initiates the reperfusion injury cascade culminating in injury and death of cells and tissues. Thus, cultured cells subjected to hypoxia, fuel deprivation and reoxygenation replicate the cardinal features of ischemia–reperfusion, while accommodating interventions such as siRNA suppression of specific genes and pharmacological activation or inhibition of signaling cascades that are not feasible in more complex preparations, especially intact animals. This chapter describes an in vitro OGD-reoxygenation cell culture model, an excellent preparation to examine the cellular mechanisms mediating ischemia–reperfusion injury and/or cytoprotection.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages229-235
Number of pages7
DOIs
StatePublished - 1 Jan 2018

Publication series

NameMethods in Molecular Biology
Volume1717
ISSN (Print)1064-3745

Fingerprint

Wounds and Injuries
Oxygen
Cytoprotection
Heart Arrest
Reperfusion Injury
Small Interfering RNA
Transcriptional Activation
Reperfusion
Cause of Death
Cultured Cells
Oxidative Stress
Cell Death
Ischemia
Cell Culture Techniques
Stroke
Myocardial Infarction
Pharmacology
Brain
In Vitro Techniques
Therapeutics

Keywords

  • Apoptosis
  • Cell culture
  • Ischemia–reperfusion (I/R) neurons
  • Oxygen–glucose deprivation (OGD)

Cite this

Ryou, M. G., & Mallet, R. T. (2018). An in vitro oxygen–glucose deprivation model for studying ischemia–reperfusion injury of neuronal cells. In Methods in Molecular Biology (pp. 229-235). (Methods in Molecular Biology; Vol. 1717). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-7526-6_18
Ryou, Myoung gwi ; Mallet, Robert T. / An in vitro oxygen–glucose deprivation model for studying ischemia–reperfusion injury of neuronal cells. Methods in Molecular Biology. Humana Press Inc., 2018. pp. 229-235 (Methods in Molecular Biology).
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Ryou, MG & Mallet, RT 2018, An in vitro oxygen–glucose deprivation model for studying ischemia–reperfusion injury of neuronal cells. in Methods in Molecular Biology. Methods in Molecular Biology, vol. 1717, Humana Press Inc., pp. 229-235. https://doi.org/10.1007/978-1-4939-7526-6_18

An in vitro oxygen–glucose deprivation model for studying ischemia–reperfusion injury of neuronal cells. / Ryou, Myoung gwi; Mallet, Robert T.

Methods in Molecular Biology. Humana Press Inc., 2018. p. 229-235 (Methods in Molecular Biology; Vol. 1717).

Research output: Chapter in Book/Report/Conference proceedingChapter

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Ryou MG, Mallet RT. An in vitro oxygen–glucose deprivation model for studying ischemia–reperfusion injury of neuronal cells. In Methods in Molecular Biology. Humana Press Inc. 2018. p. 229-235. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-7526-6_18