An evaluation of the RapidHIT® system for reliably genotyping reference samples

Bobby L. Larue; Andrea Moore; Jonathan L. King; Pamela L. Marshall; Bruce Budowle

doi:10.1016/j.fsigen.2014.06.012

An evaluation of the RapidHIT® system for reliably genotyping reference samples

Bobby L. Larue, Andrea Moore, Jonathan L. King, Pamela L. Marshall, Bruce Budowle

Research output: Contribution to journal › Article › peer-review

30 Scopus citations

Abstract

Short tandem repeat (STR) typing is used routinely for associating or excluding individuals with biological evidence left at a crime scene. Improvements have been made to reduce the turnaround time and labor involved with profile generation, but there is still some lag time between sample collection and interpretation of results. The RapidHIT® (IntegenX; Pleasanton, CA, USA) system is an automated instrument that is configured to perform DNA extraction, bead-based DNA normalization, amplification, electrophoresis of PCR amplicons, and data analysis of five reference swabs simultaneously. The RapidHIT system provided reliable STR profiles from reference buccal swabs in approximately 90 min with nominal "hands-on" sample loading time with no evidence of contamination between samples. The overall success rate of typing buccal swabs was comparable to standard typing systems. In the event of a failed run due to instrument failure, the swab can be removed from the cartridge and reanalyzed in the RapidHIT system or with standard STR genotyping workflows.

Original language	English
Pages (from-to)	104-111
Number of pages	8
Journal	Forensic Science International: Genetics
Volume	13
DOIs	https://doi.org/10.1016/j.fsigen.2014.06.012
State	Published - Nov 2014

Keywords

Automated STR typing
R-DNA
Rapid DNA
Rapid genotyping
STR

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10.1016/j.fsigen.2014.06.012

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title = "An evaluation of the RapidHIT{\textregistered} system for reliably genotyping reference samples",

abstract = "Short tandem repeat (STR) typing is used routinely for associating or excluding individuals with biological evidence left at a crime scene. Improvements have been made to reduce the turnaround time and labor involved with profile generation, but there is still some lag time between sample collection and interpretation of results. The RapidHIT{\textregistered} (IntegenX; Pleasanton, CA, USA) system is an automated instrument that is configured to perform DNA extraction, bead-based DNA normalization, amplification, electrophoresis of PCR amplicons, and data analysis of five reference swabs simultaneously. The RapidHIT system provided reliable STR profiles from reference buccal swabs in approximately 90 min with nominal {"}hands-on{"} sample loading time with no evidence of contamination between samples. The overall success rate of typing buccal swabs was comparable to standard typing systems. In the event of a failed run due to instrument failure, the swab can be removed from the cartridge and reanalyzed in the RapidHIT system or with standard STR genotyping workflows.",

keywords = "Automated STR typing, R-DNA, Rapid DNA, Rapid genotyping, STR",

author = "Larue, {Bobby L.} and Andrea Moore and King, {Jonathan L.} and Marshall, {Pamela L.} and Bruce Budowle",

note = "Funding Information: The authors would like to thank IntegenX and Diomics for material and technical support during this study. Portions of this work were performed in conjunction with training conducted by RTI International which is supported by NIJ training Grant 2011-DN-BX-K564 .",

year = "2014",

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doi = "10.1016/j.fsigen.2014.06.012",

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AU - Budowle, Bruce

N1 - Funding Information: The authors would like to thank IntegenX and Diomics for material and technical support during this study. Portions of this work were performed in conjunction with training conducted by RTI International which is supported by NIJ training Grant 2011-DN-BX-K564 .

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N2 - Short tandem repeat (STR) typing is used routinely for associating or excluding individuals with biological evidence left at a crime scene. Improvements have been made to reduce the turnaround time and labor involved with profile generation, but there is still some lag time between sample collection and interpretation of results. The RapidHIT® (IntegenX; Pleasanton, CA, USA) system is an automated instrument that is configured to perform DNA extraction, bead-based DNA normalization, amplification, electrophoresis of PCR amplicons, and data analysis of five reference swabs simultaneously. The RapidHIT system provided reliable STR profiles from reference buccal swabs in approximately 90 min with nominal "hands-on" sample loading time with no evidence of contamination between samples. The overall success rate of typing buccal swabs was comparable to standard typing systems. In the event of a failed run due to instrument failure, the swab can be removed from the cartridge and reanalyzed in the RapidHIT system or with standard STR genotyping workflows.

AB - Short tandem repeat (STR) typing is used routinely for associating or excluding individuals with biological evidence left at a crime scene. Improvements have been made to reduce the turnaround time and labor involved with profile generation, but there is still some lag time between sample collection and interpretation of results. The RapidHIT® (IntegenX; Pleasanton, CA, USA) system is an automated instrument that is configured to perform DNA extraction, bead-based DNA normalization, amplification, electrophoresis of PCR amplicons, and data analysis of five reference swabs simultaneously. The RapidHIT system provided reliable STR profiles from reference buccal swabs in approximately 90 min with nominal "hands-on" sample loading time with no evidence of contamination between samples. The overall success rate of typing buccal swabs was comparable to standard typing systems. In the event of a failed run due to instrument failure, the swab can be removed from the cartridge and reanalyzed in the RapidHIT system or with standard STR genotyping workflows.

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An evaluation of the RapidHIT® system for reliably genotyping reference samples

Abstract

Keywords

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