An agarose gel electrophoretic method for typing phosphoglucomutase-1, esterase D, or glyoxalase I

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Abstract

A conventional agarose gel electrophoretic method was described for typing phosphoglucomutase-1, esterase D, or glyoxalase I as single systems. Bloodstain extracts were absorbed into 1-mm-thick agarose gels via an application mask. The electrode wick distance was 12 cm and electrophoresis was carried out at 400 V at 6°C. The electrophoretic run times were 30 min for glyoxalase and 1 h for esterase D or phosphoglucomutase. This method is reliable and produces highly resolved band patterns. Additionally, the shorter separation times as a result of the increased voltage gradient permitted typing of more samples in a given time period compared with presently used methods. This technique requires little technical expertise and can be incorporated into the laboratory at a minimal cost.

Original languageEnglish
Pages (from-to)1216-1220
Number of pages5
JournalJournal of Forensic Sciences
Volume30
Issue number4
StatePublished - 1 Dec 1985

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