Amino acid substitutions Phe⁶⁶ → Leu and Ser¹²⁶ → Pro abolish cortisol and aldosterone synthesis by bovine cytochrome P450_11β

Keyphrases

• Amino Acid Substitution 100%
• Cortisol 100%
• Deoxycorticosterone 100%
• Cytochrome 100%
• Aldosterone Synthesis 100%
• Amino Acids 66%
• Mitochondria 66%
• Complementary DNA (cDNA) 66%
• Hydroxylation 66%
• 11β-hydroxylase Deficiency 66%
• COS Cells 66%
• Polymerase Chain Reaction 33%
• Amino 33%
• CDNA Synthesis 33%
• Androstenedione 33%
• Aldosterone Synthase 33%
• Aldosterone 33%
• Adrenal Cortex 33%
• Synthetase 33%
• Amino Acid Variation 33%
• CDNA Clone 33%
• Active Form 33%
• Purified Enzymes 33%
• Protein Sequence 33%
• Combined Technique 33%
• True Protein 33%
• Reverse Transcription 33%
• Reconstituted System 33%
• Cell Synthesis 33%

Biochemistry, Genetics and Molecular Biology

• Cytochrome 100%
• Amino Acid Substitution 100%
• Cortisol 100%
• Aldosterone 100%
• Amino Acids 75%
• Mitochondrion 50%
• Oxygenase 50%
• Hydroxylation 50%
• 11-Deoxycorticosterone 50%
• N-Terminus 25%
• Enzyme 25%
• Precursor 25%
• Peptide Sequence 25%
• Aldosterone Synthase 25%
• Molecular Cloning 25%
• Reverse Transcription 25%
• Complete Protein 25%
• Cortodoxone 25%
• Polymerase Chain Reaction 25%