Altered expression of the neuropeptide-processing enzyme carboxypeptidase E in the rat brain after global ischemia

Kunlin Jin, Steven H. Graham, Tetsuya Nagayama, Paul C. Goldsmith, David A. Greenberg, An Zhou, Roger P. Simon

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

Carboxypeptidase E, an exoprotease involved in the processing of bioactive peptides released by a regulated secretory pathway, was identified in a subtractive complementary DNA library derived from an ischemic rat brain by differential screening. In situ hybridization and immunocytochemical analysis showed the presence of carboxypeptidase E messenger RNA and protein in the cerebral cortex, thalamus, striatum, and hippocampus of a healthy rat brain. After 15 minutes of transient global ischemia followed by 8 hours of reperfusion, increased levels of carboxypeptidase E messenger RNA and protein were observed in the hippocampal CA1 and CA3 regions and in the cortex, as detected by Northern and Western blot analyses and in situ hybridization. After extended reperfusion (24 to 72 hours), both carboxypeptidase E messenger RNA and protein levels were decreased. The ischemiainduced changes in carboxypeptidase E expression suggest that this enzyme may play a role in modulating the brain's response to ischemia.

Original languageEnglish
Pages (from-to)1422-1429
Number of pages8
JournalJournal of Cerebral Blood Flow and Metabolism
Volume21
Issue number12
DOIs
StatePublished - 1 Jan 2001

Keywords

  • Carboxypeptidase E
  • Cerebral ischemia
  • Gene expression
  • Programmed cell death

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