Aerobic and Anaerobic Metabolism of Bovine Ciliary Process: Effects of Metabolic and Transport Inhibitors

Sharon C. Braunagel, Thomas Yorio

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

In the present study we have measured the oxygen consumption and lactic acid production, under aerobic and anaerobic conditions, in the bovine ciliary process epithelium (CPE) in the presence and absence of transport modifiers. Basal oxygen consumption was 8-15 ul O2 consumed/mg protein/hr and decreased by 35% when sodium was removed or ouabain was added to the media. Anaerobic metabolism as measured by lactate production was also attenuated by sodium-free or ouabain treatment. When O2 consumption was severely limited by cyanide, lactic acid production increased significantly (“Pasteur effect”), whereas 2-deoxyglucose reduced lactate formation. Both chloride-free and acetazolamide treated CPE increased their dependency on aerobic glycolysis, and this response was also observed under anaerobic conditions, suggesting the presence of an anion transport mechanism. A net lactate production was also found to occur across the aqueous epithelium under aerobic and anaerobic conditions. These results are consistent with the presence of a bicarbonate-sensitive anion transport system located in the ciliary process epithelium.

Original languageEnglish
Pages (from-to)141-148
Number of pages8
JournalJournal of Ocular Pharmacology
Volume3
Issue number2
DOIs
StatePublished - 1 Jan 1987

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Anaerobiosis
Lactic Acid
Epithelium
Ouabain
Oxygen Consumption
Anions
Sodium
Acetazolamide
Deoxyglucose
Cyanides
Glycolysis
Bicarbonates
Chlorides
Proteins

Cite this

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abstract = "In the present study we have measured the oxygen consumption and lactic acid production, under aerobic and anaerobic conditions, in the bovine ciliary process epithelium (CPE) in the presence and absence of transport modifiers. Basal oxygen consumption was 8-15 ul O2 consumed/mg protein/hr and decreased by 35{\%} when sodium was removed or ouabain was added to the media. Anaerobic metabolism as measured by lactate production was also attenuated by sodium-free or ouabain treatment. When O2 consumption was severely limited by cyanide, lactic acid production increased significantly (“Pasteur effect”), whereas 2-deoxyglucose reduced lactate formation. Both chloride-free and acetazolamide treated CPE increased their dependency on aerobic glycolysis, and this response was also observed under anaerobic conditions, suggesting the presence of an anion transport mechanism. A net lactate production was also found to occur across the aqueous epithelium under aerobic and anaerobic conditions. These results are consistent with the presence of a bicarbonate-sensitive anion transport system located in the ciliary process epithelium.",
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Aerobic and Anaerobic Metabolism of Bovine Ciliary Process : Effects of Metabolic and Transport Inhibitors. / Braunagel, Sharon C.; Yorio, Thomas.

In: Journal of Ocular Pharmacology, Vol. 3, No. 2, 01.01.1987, p. 141-148.

Research output: Contribution to journalArticle

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