Adipose-derived stem cells: Isolation, expansion and differentiation

Bruce A. Bunnell, Mette Flaat, Christine Gagliardi, Bindiya Patel, Cynthia Ripoll

Research output: Contribution to journalArticlepeer-review

509 Scopus citations


The emerging field of regenerative medicine will require a reliable source of stem cells in addition to biomaterial scaffolds and cytokine growth factors. Adipose tissue has proven to serve as an abundant, accessible and rich source of adult stem cells with multipotent properties suitable for tissue engineering and regenerative medical applications. There has been increased interest in adipose-derived stem cells (ASCs) for tissue engineering applications. Here, methods for the isolation, expansion and differentiation of ASCs are presented and described in detail. While this article has focused on the isolation of ASCs from human adipose tissue, the procedure can be applied to adipose tissues from other species with minimal modifications.

Original languageEnglish
Pages (from-to)115-120
Number of pages6
Issue number2
StatePublished - Jun 2008


  • Adipose-derived stem cells (ASCs)
  • Biopsy
  • Differentiation
  • Expansion
  • Isolation
  • Lipoaspirate
  • Mesenchymal stem cells (MSCs)


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