Adipose-derived stem cells: Isolation, expansion and differentiation

Bruce A. Bunnell; Mette Flaat; Christine Gagliardi; Bindiya Patel; Cynthia Ripoll

doi:10.1016/j.ymeth.2008.03.006

Adipose-derived stem cells: Isolation, expansion and differentiation

Bruce A. Bunnell, Mette Flaat, Christine Gagliardi, Bindiya Patel, Cynthia Ripoll

Research output: Contribution to journal › Article › peer-review

509 Scopus citations

Abstract

The emerging field of regenerative medicine will require a reliable source of stem cells in addition to biomaterial scaffolds and cytokine growth factors. Adipose tissue has proven to serve as an abundant, accessible and rich source of adult stem cells with multipotent properties suitable for tissue engineering and regenerative medical applications. There has been increased interest in adipose-derived stem cells (ASCs) for tissue engineering applications. Here, methods for the isolation, expansion and differentiation of ASCs are presented and described in detail. While this article has focused on the isolation of ASCs from human adipose tissue, the procedure can be applied to adipose tissues from other species with minimal modifications.

Original language	English
Pages (from-to)	115-120
Number of pages	6
Journal	Methods
Volume	45
Issue number	2
DOIs	https://doi.org/10.1016/j.ymeth.2008.03.006
State	Published - Jun 2008

Keywords

Adipose-derived stem cells (ASCs)
Biopsy
Differentiation
Expansion
Isolation
Lipoaspirate
Mesenchymal stem cells (MSCs)

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10.1016/j.ymeth.2008.03.006

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title = "Adipose-derived stem cells: Isolation, expansion and differentiation",

abstract = "The emerging field of regenerative medicine will require a reliable source of stem cells in addition to biomaterial scaffolds and cytokine growth factors. Adipose tissue has proven to serve as an abundant, accessible and rich source of adult stem cells with multipotent properties suitable for tissue engineering and regenerative medical applications. There has been increased interest in adipose-derived stem cells (ASCs) for tissue engineering applications. Here, methods for the isolation, expansion and differentiation of ASCs are presented and described in detail. While this article has focused on the isolation of ASCs from human adipose tissue, the procedure can be applied to adipose tissues from other species with minimal modifications.",

keywords = "Adipose-derived stem cells (ASCs), Biopsy, Differentiation, Expansion, Isolation, Lipoaspirate, Mesenchymal stem cells (MSCs)",

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note = "Funding Information: The research was supported by the National Center for Research Resources, National Institutes of Health, Grant No. RR00164, and a grant from the State of Louisiana Millennium Health Excellence Fund and the Louisiana Gene Therapy Research Consortium. ",

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AU - Bunnell, Bruce A.

AU - Flaat, Mette

AU - Gagliardi, Christine

AU - Patel, Bindiya

AU - Ripoll, Cynthia

N1 - Funding Information: The research was supported by the National Center for Research Resources, National Institutes of Health, Grant No. RR00164, and a grant from the State of Louisiana Millennium Health Excellence Fund and the Louisiana Gene Therapy Research Consortium.

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KW - Expansion

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Adipose-derived stem cells: Isolation, expansion and differentiation

Abstract

Keywords

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