Adenosine receptor mediates motility in human melanoma cells

Elisa C. Woodhouse, Derek F. Amanatullah, John Schetz, Lance A. Liotta, Mary L. Stracke, Timothy Clair

Research output: Contribution to journalArticle

50 Scopus citations

Abstract

Cell motility is an essential component of tumor progression and metastasis. A number of factors, both autocrine and paracrine, have been found to influence cell motility. In the present study, adenosine and adenine nucleotides directly stimulated chemotaxis of A2058 melanoma cells in the absence of exogenous factors. Three adenosine receptor agonists stimulated motility in the melanoma cells and two adenosine receptor antagonists strongly inhibited the chemotactic response to both adenosine and AMP. The chemotactic stimulation by adenosine and AMP was pertussis toxin sensitive. Otherwise unresponsive Chinese hamster ovary cells which were transfected with the adenosine A 1 receptor cDNA acquired the direct, pertussis toxin sensitive, chemotactic response to adenosine, and this response was inhibited by adenosine receptor antagonists. These findings demonstrate that adenosine and adenine nucleotides are capable of stimulating chemotaxis of tumor cells mediated through an adenosine receptor, probably of the A 1 subtype. The possibility of antimetastatic therapies based on inhibition of adenosine receptor activity is raised.

Original languageEnglish
Pages (from-to)888-894
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume246
Issue number3
DOIs
StatePublished - 29 May 1998

Keywords

  • Adenosine
  • Adenosine receptor
  • Chemotaxis
  • Melanoma

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    Woodhouse, E. C., Amanatullah, D. F., Schetz, J., Liotta, L. A., Stracke, M. L., & Clair, T. (1998). Adenosine receptor mediates motility in human melanoma cells. Biochemical and Biophysical Research Communications, 246(3), 888-894. https://doi.org/10.1006/bbrc.1998.8714