TY - JOUR
T1 - Adaptive changes of H+ secreting cells in the epidermis of the leopard frog RANA PIPIENS
AU - Page, Ray D.
AU - Mia, A. J.
AU - Buttar, S.
AU - Yorio, Thomas
N1 - Funding Information:
Acknowledgemenrs-Threiss earcwh as supporteidn part from a grant from the Texas ResearchE nhancemenPt ro-gram( TY) andN HLBI K14HL01730(A JM). Ray Pagew as &pported by an AOA/Burroughs Wellcomed steoiathic ResearchF ellowshin( F89-08).T he authorsw ould like to thank Dr L. X. Oakford for assistancew ith quantification of the MR cells by light microscopy, and Christina Ernemannf or technicala ssistance.
PY - 1990
Y1 - 1990
N2 - 1. Mitochondria-rich (MR) cells in the integument of the southern leopard frog, Rana pipiens, berlandieri, were stained with AgNO3 under a variety of environmental and metabolic treatment conditions known to increase H+ excretion rates across the skin. In this tissue AgNO3 proved to be a good stain for discriminating the MR cell populations from the granular cells. 2. High salinity adapted southern frogs showed no change in the MR cell population. The inability of the MR cell number to significantly increase suggested that the increased H+ excretion rates previously seen in these animals were not due to increased MR cell proliferation. 3. The MR cell population was found to increase in the NaNO3 adapted frogs, demonstrating the contribution of altered extracellular Cl- concentrations on the regulation of MR cell density. 4. Animals that were placed in chronic metabolic acidosis or pre-treated with ibuprofen demonstrated an increased MR cell population. The current observations are consistent with previous findings that these treatment regimes increase H+ excretion, suggesting that one of the cellular adaptive mechanisms responsible for increasing H+ excretion involves increasing the MR cell density. 5. The results further suggest that prostaglandins may play a role in regulating H+ excretion in MR cells, and that either changes in intracellular pH or prostaglandin formation regulates cell proliferation.
AB - 1. Mitochondria-rich (MR) cells in the integument of the southern leopard frog, Rana pipiens, berlandieri, were stained with AgNO3 under a variety of environmental and metabolic treatment conditions known to increase H+ excretion rates across the skin. In this tissue AgNO3 proved to be a good stain for discriminating the MR cell populations from the granular cells. 2. High salinity adapted southern frogs showed no change in the MR cell population. The inability of the MR cell number to significantly increase suggested that the increased H+ excretion rates previously seen in these animals were not due to increased MR cell proliferation. 3. The MR cell population was found to increase in the NaNO3 adapted frogs, demonstrating the contribution of altered extracellular Cl- concentrations on the regulation of MR cell density. 4. Animals that were placed in chronic metabolic acidosis or pre-treated with ibuprofen demonstrated an increased MR cell population. The current observations are consistent with previous findings that these treatment regimes increase H+ excretion, suggesting that one of the cellular adaptive mechanisms responsible for increasing H+ excretion involves increasing the MR cell density. 5. The results further suggest that prostaglandins may play a role in regulating H+ excretion in MR cells, and that either changes in intracellular pH or prostaglandin formation regulates cell proliferation.
UR - http://www.scopus.com/inward/record.url?scp=0025064958&partnerID=8YFLogxK
U2 - 10.1016/0742-8413(90)90004-S
DO - 10.1016/0742-8413(90)90004-S
M3 - Article
C2 - 1980436
AN - SCOPUS:0025064958
SN - 0306-4492
VL - 96
SP - 245
EP - 251
JO - Comparative Biochemistry and Physiology. Part C, Comparative
JF - Comparative Biochemistry and Physiology. Part C, Comparative
IS - 2
ER -