TY - JOUR
T1 - Acute effects of glaucoma medications on rat intraocular pressure
AU - Pang, Iok-Hou
AU - Wang, Wan Heng
AU - Clark, Abbot
PY - 2005/2/1
Y1 - 2005/2/1
N2 - The rat has been used increasingly in glaucoma research, but many aspects regarding the regulation of its intraocular pressure (IOP) are still unknown. For example, it is not clear whether glaucoma medications can lower IOP in the rat similarly to human. This information will be valuable in evaluating this animal model for its usefulness in predicting drug effects in patients. Hence, we tested the acute IOP effects of selected glaucoma drugs topical administered onto the rat eye. In these studies, IOP was measured using the Tono-Pen XL ® tonometer. After a correlation between the IOP reported by the Tono-Pen and actual IOP was established, IOP measurements were obtained in slightly sedated adult rats. Effects of glaucoma medications were tested in two groups of animals. One group (12 h/L) was housed in a 12-h/12-h light/dark cycle. The other (24 h/L) was housed under constant light. Exposure of the animals to constant light increased their basal IOP from 20.5±0.6 mmHg (mean±s.e.m., n=12) to 32.0±0.5 mmHg. At 3 h after topical administration, Betoptic S® lowered IOP by 4.3±1.7 mmHg (n=6) and 3.7±0.3 mmHg (n=6) in the 12 and 24 h/L rats, respectively. Pilocarpine did not affect rat IOP. Xalatan® produced a biphasic response in the rat. At 3 h after topical administration, it increased IOP by 7.9±1.4 and 7.0±1.0 mmHg in the 12 and 24 h/L rats, respectively. By the next day, it decreased IOP by 3.0±1.0 and 6.0±0.8 mmHg in the 12 and 24 h/L rats, respectively. The IOP-enhancing effect of Xalatan was dose-dependent. The present study indicates that IOP responses of the rat to different pharmacological agents are not identical to those of the human. In the rat, Betoptic S, but not pilocarpine, lowered IOP. Xalatan initially increased then decreased IOP.
AB - The rat has been used increasingly in glaucoma research, but many aspects regarding the regulation of its intraocular pressure (IOP) are still unknown. For example, it is not clear whether glaucoma medications can lower IOP in the rat similarly to human. This information will be valuable in evaluating this animal model for its usefulness in predicting drug effects in patients. Hence, we tested the acute IOP effects of selected glaucoma drugs topical administered onto the rat eye. In these studies, IOP was measured using the Tono-Pen XL ® tonometer. After a correlation between the IOP reported by the Tono-Pen and actual IOP was established, IOP measurements were obtained in slightly sedated adult rats. Effects of glaucoma medications were tested in two groups of animals. One group (12 h/L) was housed in a 12-h/12-h light/dark cycle. The other (24 h/L) was housed under constant light. Exposure of the animals to constant light increased their basal IOP from 20.5±0.6 mmHg (mean±s.e.m., n=12) to 32.0±0.5 mmHg. At 3 h after topical administration, Betoptic S® lowered IOP by 4.3±1.7 mmHg (n=6) and 3.7±0.3 mmHg (n=6) in the 12 and 24 h/L rats, respectively. Pilocarpine did not affect rat IOP. Xalatan® produced a biphasic response in the rat. At 3 h after topical administration, it increased IOP by 7.9±1.4 and 7.0±1.0 mmHg in the 12 and 24 h/L rats, respectively. By the next day, it decreased IOP by 3.0±1.0 and 6.0±0.8 mmHg in the 12 and 24 h/L rats, respectively. The IOP-enhancing effect of Xalatan was dose-dependent. The present study indicates that IOP responses of the rat to different pharmacological agents are not identical to those of the human. In the rat, Betoptic S, but not pilocarpine, lowered IOP. Xalatan initially increased then decreased IOP.
KW - betaxolol
KW - intraocular pressure
KW - latanoprost
KW - pilocarpine
KW - rat
UR - http://www.scopus.com/inward/record.url?scp=12744250272&partnerID=8YFLogxK
U2 - 10.1016/j.exer.2004.09.001
DO - 10.1016/j.exer.2004.09.001
M3 - Article
C2 - 15670799
AN - SCOPUS:12744250272
SN - 0014-4835
VL - 80
SP - 207
EP - 214
JO - Experimental Eye Research
JF - Experimental Eye Research
IS - 2
ER -