Activation of NF-κB by the full-length nucleocapsid protein of the SARS coronavirus

Qing Jiao Liao, Lin Bai Ye, Khalid Amine Timani, Ying Chun Zeng, Ying Long She, Li Ye, Zheng Hui Wu

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21 Scopus citations

Abstract

The severe acute respiratory syndrome coronavirus (SARS-CoV) is the major causative agent for the worldwide outbreak of SARS in 2003. The mechanism by which SARS-CoV causes atypical pneumonia remains unclear. The nuclear factor kappa B (NF-κB) is a key transcription factor that activates numerous genes involved in cellular immune response and inflammation. Many studies have shown that NF-κB plays an important role in the pathogenesis of lung diseases. In this study, we investigated the possible regulatory interaction between the SARS-CoV nucleocapsid (N) protein and NF-κB by luciferase activity assay. Our results showed that the SARS-CoV N protein can significantly activate NF-κB only in Vero E6 cells, which are susceptible to SARS-CoV infection, but not in Vero or HeLa cells. This suggests that NF-κB activation is cell-specific. Furthermore, NF-κB activation in Vero E6 cells expressing the N protein is dose-dependent. Further experiments showed that there is more than one function domain in the N protein responsible for NF-κB activation. Our data indicated the possible role of the N protein in the pathogenesis of SARS.

Original languageEnglish
Pages (from-to)607-612
Number of pages6
JournalActa Biochimica et Biophysica Sinica
Volume37
Issue number9
DOIs
Publication statusPublished - 1 Sep 2005

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Keywords

  • NF-κB
  • Nucleocapsid protein
  • Severe acute respiratory syndrome coronavirus (SARS-CoV)

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