TY - JOUR
T1 - Activation of murine epidermal γδ T cells through surface 2B4
AU - Schuhmachers, Georg
AU - Ariizumi, Kiyoshi
AU - Mathew, Porunelloor A.
AU - Bennett, Michael
AU - Kumar, Yinay
AU - Takashima, Akira
PY - 1995/4
Y1 - 1995/4
N2 - Dendritic epidermal T cells (DETC) are γδ T cells that normally reside in murine skin. They express on their surface the 2B4 molecule, a 66‐kDa glycoprotein of the immunoglobulin gene superfamily thought to be associated with anti‐tumor cytotoxicity by natural killer and lymphokine‐activated killer cells. Here, we show that ligation of surface 2B4 transduces cell activation signals in DETC. Treatment with anti‐2B4 monoclonal antibodies triggers the secretion of interferon‐γ and interleukin‐2 by DETC lines, induces proliferation of resting DETC lines, amplifies anti‐CD3‐dependent proliferation of DETC freshly isolated from mouse skin; and up‐regulates egr‐1 and c‐fos mRNA expression. These results indicate a unique pathway for DETC activation.
AB - Dendritic epidermal T cells (DETC) are γδ T cells that normally reside in murine skin. They express on their surface the 2B4 molecule, a 66‐kDa glycoprotein of the immunoglobulin gene superfamily thought to be associated with anti‐tumor cytotoxicity by natural killer and lymphokine‐activated killer cells. Here, we show that ligation of surface 2B4 transduces cell activation signals in DETC. Treatment with anti‐2B4 monoclonal antibodies triggers the secretion of interferon‐γ and interleukin‐2 by DETC lines, induces proliferation of resting DETC lines, amplifies anti‐CD3‐dependent proliferation of DETC freshly isolated from mouse skin; and up‐regulates egr‐1 and c‐fos mRNA expression. These results indicate a unique pathway for DETC activation.
KW - 2B4
KW - Dendritic epidermal T cells
KW - Signaling
UR - http://www.scopus.com/inward/record.url?scp=0028956698&partnerID=8YFLogxK
U2 - 10.1002/eji.1830250440
DO - 10.1002/eji.1830250440
M3 - Article
C2 - 7737283
AN - SCOPUS:0028956698
SN - 0014-2980
VL - 25
SP - 1117
EP - 1120
JO - European Journal of Immunology
JF - European Journal of Immunology
IS - 4
ER -