Acrylmide quenching of Yt-base fluorescence in aqueous solution

Ignacy Gryczynski, Michael L. Johnson, Joseph R. Lakowicz

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Acrylamide was found to be an effective quencher of Yt-base (Y-4,9-dihydro-4,6-dimethyl-9-oxo-1H-imidazo-1,2a-purine) in water. In the absence of collisional quenching the decay of Yt-base in water is predominantly a single exponential. The intensity decays become increasingly heterogeneous when quenched by acrylamide. The frequency-domain data were analyzed using the radiation model, which provides estimates of molecular parameters characteristic of the system. The mutual diffusion coefficient at 20 ° C was found to be 0.5 × 105cm2/s, the Yt-base acrylamide interaction radius was 8 Å, and the rate constant for quenching was 100 cm/s. These values indicate that quenching is diffusion-limited, i.e., the encounter complex is deactivated at least 2-fold faster than the rate of diffusive encounters.

Original languageEnglish
Pages (from-to)269-274
Number of pages6
JournalBiophysical Chemistry
Volume31
Issue number3
DOIs
StatePublished - Sep 1988

Keywords

  • Fluorescence quenching
  • Frequency-domain fluorometry
  • Lifetime decay
  • Transient effect

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