Abundance of TRPC6 protein in glomerular mesangial cells is decreased by ROS and PKC in diabetes

Sarabeth Graham, Yves Gorin, Hanna E. Abboud, Min Ding, Duck Yoon Lee, Honglian Shi, Yanfeng Ding, Rong Ma

Research output: Contribution to journalArticle

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Abstract

The present study was performed to investigate the underlying mechanism, particularly the roles of reactive oxygen species (ROS) and protein kinase C (PKC), in the diabetes-induced canonical transient receptor potential 6 (TRPC6) downregulation. We found that high glucose (HG) significantly reduced TRPC6 protein expression in cultured mesangial cells (MCs). TRPC6 protein was also significantly reduced in the glomeruli but not in the heart or aorta isolated from streptozotocin-induced diabetic rats. In the cultured MCs, H 2O 2 suppressed TRPC6 protein expression in a dose- and time-dependent manner, which emulated the HG effect. Catalase as well as superoxide dismutase were able to prevent the inhibitory effect of HG on TRPC6. The antioxidant effect observed in cultured cells was also observed in diabetic rats treated with tempol for 2 wk, which exhibited a preservation of TRPC6 in the glomeruli. Specific knockdown of Nox4, a component of NADPH oxidase, increased TRPC6 protein expression. Furthermore, the PKC activator phorbol 12-myristate 13-acetate (PMA), but not its analog 4α-phorbol 12, 13-didecanoate (4α-PDD), suppressed TRPC6 expression, and this PMA effect was not affected by catalase. Moreover, Gö6976, but not LY333531, attenuated the negative effect of HG on TRPC6 expression. Gö6976 also inhibited H 2O 2 effect on TRPC6. Furthermore, either knockdown of TRPC6 or HG treatment significantly decreased ANG II-stimulated MC contraction, and the HGimpaired MC contraction was rescued by overexpression of TRPC6. These results suggest that hyperglycemia in diabetes downregulated TRPC6 protein expression in MCs through a NADPH oxidase Nox4- ROS-PKC pathway, proving a mechanism for impaired MC contraction in diabetes.

Original languageEnglish
Pages (from-to)C304-C315
JournalAmerican Journal of Physiology - Cell Physiology
Volume301
Issue number2
DOIs
StatePublished - 1 Aug 2011

Fingerprint

Mesangial Cells
Protein Kinase C
Reactive Oxygen Species
Glucose
Cultured Cells
Proteins
NADPH Oxidase
Catalase
ruboxistaurin
Acetates
Down-Regulation
Streptozocin
Hyperglycemia
Superoxide Dismutase
Aorta
Antioxidants

Keywords

  • Calcium ion channel
  • Diabetic nephropathy
  • Oxidative stress

Cite this

Graham, Sarabeth ; Gorin, Yves ; Abboud, Hanna E. ; Ding, Min ; Lee, Duck Yoon ; Shi, Honglian ; Ding, Yanfeng ; Ma, Rong. / Abundance of TRPC6 protein in glomerular mesangial cells is decreased by ROS and PKC in diabetes. In: American Journal of Physiology - Cell Physiology. 2011 ; Vol. 301, No. 2. pp. C304-C315.
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abstract = "The present study was performed to investigate the underlying mechanism, particularly the roles of reactive oxygen species (ROS) and protein kinase C (PKC), in the diabetes-induced canonical transient receptor potential 6 (TRPC6) downregulation. We found that high glucose (HG) significantly reduced TRPC6 protein expression in cultured mesangial cells (MCs). TRPC6 protein was also significantly reduced in the glomeruli but not in the heart or aorta isolated from streptozotocin-induced diabetic rats. In the cultured MCs, H 2O 2 suppressed TRPC6 protein expression in a dose- and time-dependent manner, which emulated the HG effect. Catalase as well as superoxide dismutase were able to prevent the inhibitory effect of HG on TRPC6. The antioxidant effect observed in cultured cells was also observed in diabetic rats treated with tempol for 2 wk, which exhibited a preservation of TRPC6 in the glomeruli. Specific knockdown of Nox4, a component of NADPH oxidase, increased TRPC6 protein expression. Furthermore, the PKC activator phorbol 12-myristate 13-acetate (PMA), but not its analog 4α-phorbol 12, 13-didecanoate (4α-PDD), suppressed TRPC6 expression, and this PMA effect was not affected by catalase. Moreover, G{\"o}6976, but not LY333531, attenuated the negative effect of HG on TRPC6 expression. G{\"o}6976 also inhibited H 2O 2 effect on TRPC6. Furthermore, either knockdown of TRPC6 or HG treatment significantly decreased ANG II-stimulated MC contraction, and the HGimpaired MC contraction was rescued by overexpression of TRPC6. These results suggest that hyperglycemia in diabetes downregulated TRPC6 protein expression in MCs through a NADPH oxidase Nox4- ROS-PKC pathway, proving a mechanism for impaired MC contraction in diabetes.",
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Abundance of TRPC6 protein in glomerular mesangial cells is decreased by ROS and PKC in diabetes. / Graham, Sarabeth; Gorin, Yves; Abboud, Hanna E.; Ding, Min; Lee, Duck Yoon; Shi, Honglian; Ding, Yanfeng; Ma, Rong.

In: American Journal of Physiology - Cell Physiology, Vol. 301, No. 2, 01.08.2011, p. C304-C315.

Research output: Contribution to journalArticle

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AU - Graham, Sarabeth

AU - Gorin, Yves

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