TY - JOUR
T1 - A white-specific gene in the white-opaque switching system of Candida albicans
AU - Srikantha, T.
AU - Soll, D. R.
N1 - Funding Information:
We thank Drs. W.A. Fonzi, P. Sundstrom,a nd P.A. Sypherd for a generousg ift of EFla2 gene probe; B. Morrow for providing 3153AR NA samplesM; . Rotman for technicals upport;B . Padanilamf or helpingi n Swiss Prot analysisu sing the GCG program;K . Wheelera nd S. Harger for typing the manuscript;a nd all of the lab colleaguesf or useful discussions.T his work was supported by Public Health ServiceG rant Al2392 from the National Instituteso f Health.
PY - 1993/9/6
Y1 - 1993/9/6
N2 - The Candida albicans strain WO-1 cells switch spontaneously, frequently, and reversibly between white and opaque colony-forming units which differ dramatically in their budding phenotypes. By screening a subtracted white cDNA library, the first white-specific cDNA, cWh11, was isolated and sequenced. This, in turn, was used to clone the Wh11 gene. Wh11 shows significant homology to the glucose/lipid-regulated GLP1 gene of Saccharomyces cerevisiae. Upon temperature-induced mass conversion from opaque to white colonies, transcription of Wh11 is abruptly activated at the second cell doubling, concomitant with commitment to the white phenotype. Wh11 exhibits the unique characteristics of being regulated not only by switching, but also by the bud-hypha transition.
AB - The Candida albicans strain WO-1 cells switch spontaneously, frequently, and reversibly between white and opaque colony-forming units which differ dramatically in their budding phenotypes. By screening a subtracted white cDNA library, the first white-specific cDNA, cWh11, was isolated and sequenced. This, in turn, was used to clone the Wh11 gene. Wh11 shows significant homology to the glucose/lipid-regulated GLP1 gene of Saccharomyces cerevisiae. Upon temperature-induced mass conversion from opaque to white colonies, transcription of Wh11 is abruptly activated at the second cell doubling, concomitant with commitment to the white phenotype. Wh11 exhibits the unique characteristics of being regulated not only by switching, but also by the bud-hypha transition.
KW - GLP1homology
KW - Subtraction cDNA library
KW - buds
KW - dimorphism
KW - genomic library
KW - hypha
KW - mass phenotypic conversion
KW - transcriptional regulation
UR - http://www.scopus.com/inward/record.url?scp=0027305564&partnerID=8YFLogxK
U2 - 10.1016/0378-1119(93)90668-S
DO - 10.1016/0378-1119(93)90668-S
M3 - Article
C2 - 7916716
AN - SCOPUS:0027305564
SN - 0378-1119
VL - 131
SP - 53
EP - 60
JO - Gene
JF - Gene
IS - 1
ER -