A validation study of the Qiagen Investigator DIPplex® kit; An INDEL-based assay for human identification

Bobby L. LaRue, Jianye Ge, Jonathan L. King, Bruce Budowle

Research output: Contribution to journalArticle

68 Scopus citations

Abstract

Marker sets that are based on small insertion/deletion (INDEL) alleles can serve as useful supplementary or stand-alone assays for human identification. Avalidation study has been performed on a human identification assay based on a panel of 30 INDELs and amelogenin using the Investigator DIPplex® kit (Qiagen). The assay was able to type DNA from a number of forensically relevant sample types and obtain full profiles with 62 pg of template DNA and partial profiles with as little as 16 pg of template DNA. The assay is reproducible, precise, and non-overlapping alleles from minor contributors were detectable in mixture analysis ranging from 6:1 to 19:1 mixtures. Population studies were performed on the 30 indels, and there were no significant departures from Hardy-Weinberg equilibrium or significant linkage disequilibrium between the markers (after correction for sampling). In all populations, the random match probability was 1.43×10 -11 or less, and the power of exclusion was greater than .999999999. We also discovered several microvariant alleles in our population samples. The data support that the Investigator DIPplex® kit provides a powerful supplement or stand-alone capability for human identity testing.

Original languageEnglish
Pages (from-to)533-540
Number of pages8
JournalInternational journal of legal medicine
Volume126
Issue number4
DOIs
StatePublished - 1 Jul 2012

Keywords

  • Degraded DNA
  • Forensic DNA analysis
  • Genotyping
  • INDEL
  • Population genetics
  • SNP
  • STR
  • Validation

Fingerprint Dive into the research topics of 'A validation study of the Qiagen Investigator DIPplex® kit; An INDEL-based assay for human identification'. Together they form a unique fingerprint.

  • Cite this