A survey of the impact of deyolking on biological processes covered by shotgun proteomic analyses of zebrafish embryos

Fatima Rahlouni, Szabolcs Szarka, Vladimir Shulaev, Laszlo Prokai

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Deyolking, the removal of the most abundant protein from the zebrafish (Danio rerio) embryo, is a common technique for in-depth exploration of proteome-level changes in vivo due to various environmental stressors or pharmacological impacts during embryonic stage of development. However, the effect of this procedure on the remaining proteome has not been fully studied. Here, we report a label-free shotgun proteomics survey on proteome coverage and biological processes that are enriched and depleted as a result of deyolking. Enriched proteins are involved in cellular energetics and development pathways, specifically implicating enrichment related to mitochondrial function. Although few proteins were removed completely by deyolking, depleted molecular pathways were associated with calcium signaling and signaling events implicating immune system response.

Original languageEnglish
Pages (from-to)398-407
Number of pages10
JournalZebrafish
Volume12
Issue number6
DOIs
StatePublished - 1 Dec 2015

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Biological Phenomena
Firearms
Zebrafish
Proteome
proteome
Danio rerio
Proteomics
proteomics
embryo (animal)
Embryonic Structures
Zebrafish Proteins
Calcium Signaling
proteins
Embryonic Development
immune system
Immune System
Proteins
Pharmacology
Surveys and Questionnaires
methodology

Cite this

Rahlouni, Fatima ; Szarka, Szabolcs ; Shulaev, Vladimir ; Prokai, Laszlo. / A survey of the impact of deyolking on biological processes covered by shotgun proteomic analyses of zebrafish embryos. In: Zebrafish. 2015 ; Vol. 12, No. 6. pp. 398-407.
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A survey of the impact of deyolking on biological processes covered by shotgun proteomic analyses of zebrafish embryos. / Rahlouni, Fatima; Szarka, Szabolcs; Shulaev, Vladimir; Prokai, Laszlo.

In: Zebrafish, Vol. 12, No. 6, 01.12.2015, p. 398-407.

Research output: Contribution to journalArticleResearchpeer-review

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