TY - JOUR
T1 - A survey of the impact of deyolking on biological processes covered by shotgun proteomic analyses of zebrafish embryos
AU - Rahlouni, Fatima
AU - Szarka, Szabolcs
AU - Shulaev, Vladimir
AU - Prokai, Laszlo
N1 - Publisher Copyright:
© Mary Ann Liebert, Inc. 2015.
PY - 2015/12
Y1 - 2015/12
N2 - Deyolking, the removal of the most abundant protein from the zebrafish (Danio rerio) embryo, is a common technique for in-depth exploration of proteome-level changes in vivo due to various environmental stressors or pharmacological impacts during embryonic stage of development. However, the effect of this procedure on the remaining proteome has not been fully studied. Here, we report a label-free shotgun proteomics survey on proteome coverage and biological processes that are enriched and depleted as a result of deyolking. Enriched proteins are involved in cellular energetics and development pathways, specifically implicating enrichment related to mitochondrial function. Although few proteins were removed completely by deyolking, depleted molecular pathways were associated with calcium signaling and signaling events implicating immune system response.
AB - Deyolking, the removal of the most abundant protein from the zebrafish (Danio rerio) embryo, is a common technique for in-depth exploration of proteome-level changes in vivo due to various environmental stressors or pharmacological impacts during embryonic stage of development. However, the effect of this procedure on the remaining proteome has not been fully studied. Here, we report a label-free shotgun proteomics survey on proteome coverage and biological processes that are enriched and depleted as a result of deyolking. Enriched proteins are involved in cellular energetics and development pathways, specifically implicating enrichment related to mitochondrial function. Although few proteins were removed completely by deyolking, depleted molecular pathways were associated with calcium signaling and signaling events implicating immune system response.
UR - http://www.scopus.com/inward/record.url?scp=84949752278&partnerID=8YFLogxK
U2 - 10.1089/zeb.2015.1121
DO - 10.1089/zeb.2015.1121
M3 - Article
C2 - 26439676
AN - SCOPUS:84949752278
SN - 1545-8547
VL - 12
SP - 398
EP - 407
JO - Zebrafish
JF - Zebrafish
IS - 6
ER -