TY - JOUR
T1 - A study of the dynamic properties of actomyosin systems by quasi-elastic light scattering
AU - Hochberg, Amiram
AU - Low, William
AU - Tirosh, Reuven
AU - Borejdo, Julian
AU - Oplatka, Avraham
N1 - Funding Information:
This work was supportedin part by a grantf rom the MuscularD ystrophy Associationo f America.
PY - 1977/5/11
Y1 - 1977/5/11
N2 - A laser light source and a digital autocorrelator were employed in the study of the molecular dynamics of acto-heavy meromyosin during the splitting of ATP. Low protein concentrations were used, so that molecular and not gel properties were evident. The addition of Mg2+ to acto-heavy meromyosin solutions in the presence of ATP caused a marked widening of the spectrum at high scattering angles. No such change was observed when chemically inactivated heavy meromyosin was used, when actin was cross-linked or when the proteins were in a high ionic strength solution. The data can be interpreted in terms of pronounced change in flexibility of acto-heavy meromyosin induced by active mechanochemical coupling.
AB - A laser light source and a digital autocorrelator were employed in the study of the molecular dynamics of acto-heavy meromyosin during the splitting of ATP. Low protein concentrations were used, so that molecular and not gel properties were evident. The addition of Mg2+ to acto-heavy meromyosin solutions in the presence of ATP caused a marked widening of the spectrum at high scattering angles. No such change was observed when chemically inactivated heavy meromyosin was used, when actin was cross-linked or when the proteins were in a high ionic strength solution. The data can be interpreted in terms of pronounced change in flexibility of acto-heavy meromyosin induced by active mechanochemical coupling.
UR - http://www.scopus.com/inward/record.url?scp=0017330276&partnerID=8YFLogxK
U2 - 10.1016/0005-2728(77)90217-1
DO - 10.1016/0005-2728(77)90217-1
M3 - Article
C2 - 139920
AN - SCOPUS:0017330276
SN - 0005-2728
VL - 460
SP - 308
EP - 317
JO - Biochimica et Biophysica Acta - Bioenergetics
JF - Biochimica et Biophysica Acta - Bioenergetics
IS - 2
ER -