A rapid technique for the visualization of cytoskeletal elements using cationized ferritin

Avri Ben-ze'ev, Julian Borejdo, Avraham Oplatka

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Addition of cationized ferritin to Triton X-100 extracted fibroblasts enhances manyfold the visibility of actin filaments, intermediate filaments and microtubules. Cationized ferritin also increases the phase image of actin filaments in skeletal muscle myofibrils from which myosin has been removed, and the phase contrast of Z-bands after extracting both myosin and actin from myofibrils. It is suggested to employ this non-specific reagent as a rapid technique for the visualization of cytoskeletal elements in cells.

Original languageEnglish
Pages (from-to)1127-1135
Number of pages9
JournalCell Biology International Reports
Volume5
Issue number12
DOIs
Publication statusPublished - Dec 1981

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